SM1214RT Fibroblasts / Epithelial Cells antibody

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25 Tests / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Fibroblasts / Epithelial Cells D7-FIB

SM1214RT

Product Description for Fibroblasts / Epithelial Cells

Mouse anti Human Fibroblasts / Epithelial Cells D7-FIB.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for Fibroblasts / Epithelial Cells

Product Category Primary Antibodies
Quantity 25 Tests
Synonyms Fibroblast Marker, Fibroblasten
Presentation PE
Reactivity Hu
Applications F
Clonality Monoclonal
Clone D7-FIB
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human foreskin Fibroblasts.
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Application Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
This product is routinely tested in Flow Cytometry on the KG1 cell line.
Background A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.
Product Citations
Purchased from Acris:
Unconjugated antibody is cited in:
  1. Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB. Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. J Immunol. 2007 May 1;178(9):5552-62. PubMed PMID: 17442937.
General Readings
  1. Fearns C, Dowdle EB. The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int J Cancer. 1992 Feb 20;50(4):621-7. PubMed PMID: 1537627.
  2. Kelynack KJ, Hewitson TD, Nicholls KM, Darby IA, Becker GJ. Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process. Nephrol Dial Transplant. 2000 Nov;15(11):1766-72. PubMed PMID: 11071963.
  3. van Osch GJ, van der Veen SW, Marijnissen WJ, Verhaar JA. Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage. Biochem Biophys Res Commun. 2001 Jan 26;280(3):806-12. PubMed PMID: 11162592.
  4. Behl,B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells. Biomaterials. pii: S0142-9612(13)00039-2.
  5. Morito T, Muneta T, Hara K, Ju YJ, Mochizuki T, Makino H, et al. Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans. Rheumatology (Oxford). 2008 Aug;47(8):1137-43. doi: 10.1093/rheumatology/ken114. Epub 2008 Apr 5. PubMed PMID: 18390894.
  6. Pountos I, Giannoudis PV, Jones E, English A, Churchman S, Field S, et al. NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man. J Cell Mol Med. 2011 Mar;15(3):525-34. doi: 10.1111/j.1582-4934.2010.01006.x. PubMed PMID: 20070439.
  7. Telfer JF, Brock JH. Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium. Ann Rheum Dis. 2002 Aug;61(8):741-4. PubMed PMID: 12117685. (Free PMC Article available, 2 images available)
  8. English A, Jones EA, Corscadden D, Henshaw K, Chapman T, Emery P, et al. A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis. Rheumatology (Oxford). 2007 Nov;46(11):1676-83. Epub 2007 Sep 26. PubMed PMID: 17901063.
  9. Jones EA, English A, Kinsey SE, Straszynski L, Emery P, Ponchel F, et al. Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin Cytom. 2006 Nov 15;70(6):391-9. PubMed PMID: 16977637.
  10. Nimura A, Muneta T, Koga H, Mochizuki T, Suzuki K, Makino H, et al. Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum. Arthritis Rheum. 2008 Feb;58(2):501-10. doi: 10.1002/art.23219. PubMed PMID: 18240254.
  11. Miranda-Carús ME, Balsa A, Benito-Miguel M, De Ayala CP, Martín-Mola E. Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active. Arthritis Rheum. 2004 Sep;50(9):2786-93. PubMed PMID: 15457446.
  12. Petrow PK, Wernicke D, Schulze Westhoff C, Hummel KM, Bräuer R, Kriegsmann J, et al. Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis. Ann Rheum Dis. 2002 May;61(5):391-7. PubMed PMID: 11959761. (Free PMC Article available, 6 images available)
  13. Sekiya I, Ojima M, Suzuki S, Yamaga M, Horie M, Koga H, et al. Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis. J Orthop Res. 2012 Jun;30(6):943-9. doi: 10.1002/jor.22029. Epub 2011 Dec 6. PubMed PMID: 22147634.
  14. Kanayama M, Kurotaki D, Morimoto J, Asano T, Matsui Y, Nakayama Y, et al. Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis. J Immunol. 2009 Jun 15;182(12):8015-25. doi: 10.4049/jimmunol.0900725. PubMed PMID: 19494327.
  15. Pap T, Claus A, Ohtsu S, Hummel KM, Schwartz P, Drynda S, et al. Osteoclast-independent bone resorption by fibroblast-like cells. Arthritis Res Ther. 2003;5(3):R163-73. Epub 2003 Mar 26. PubMed PMID: 12723988. (Free PMC Article available, 5 images available)
  16. Shi Y, Niedzinski JR, Samaniego A, Bogdansky S, Atkinson BL. Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration. Tissue Eng Part A. 2012 Jul;18(13-14):1313-21. doi: 10.1089/ten.TEA.2011.0357. Epub 2012 Jun 12. PubMed PMID: 22500696.
  17. Scut, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments. J Orthop Surg Res. 3: 32.
  18. Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, et al. Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum. 2002 Dec;46(12):3349-60. PubMed PMID: 12483742.
  19. Schminke, B. et al. (2014) The Pathology of Bone Tissue during Peri-Implantitis J Dent Res. pii: 0022034514559128.
Storage Store the antibody undiluted Prior to and After reconstitution at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R. Phycoerythrin (RPE)
PE
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA, 5% Sucrose
Purity:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
State:
Lyophilized purified IgG fraction from Tissue Culture Supernatant
Reconstitution:
Restore with 0.25 ml (SM1214RT) or 1 ml (SM1214R) distilled water.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes a ~112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes.
Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes. (3). 
Negative Species: Mouse, Rat.

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