NB600-777 Fibroplasts / Epithelial Cells antibody

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Mouse anti Human Fibroplasts / Epithelial Cells D7-FIB

Product Description for Fibroplasts / Epithelial Cells

Mouse anti Human Fibroplasts / Epithelial Cells D7-FIB.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunoprecipitation, Paraffin Sections.

Properties for Fibroplasts / Epithelial Cells

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Fibroblast Marker, Fibroblasten
Presentation Purified
Reactivity Hu
Applications C, F, IP, P
Clonality Monoclonal
Clone D7-FIB
Host Mouse
Isotype IgG2a
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Human foreskin fibroblasts
Isotype control AM03096PU-N
Application flow cytometry / FACS analysis, Immunohistochemistry-Frozen, immunoprecipitation, FACS: Use 10ul of the suggested working dilution to label 1E6 cells in 100ul. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user., flow cytometry / FACS analysis 1/50-1/200, immunoprecipitation , Immunohistochemistry-Frozen 1/100
Concentration 1 mg/ml
General Readings General / background references:Fearns C & Dowdle EB The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int J Cancer 50:621-7 (1992). PubMed PMID: 1537627
Veenstra H & Dowdle EB Multi-well cell monolayers for immunocytochemistry. An alternative to cytocentrifuge preparations. J Immunol Methods 146:257-8 (1992). PubMed PMID: 1538147
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.09% Sodium Azide
protein G purified
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Human, Mouse (-), Rat (-)

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