discontinued

AM31463PU-N Filamin (90 kDa) antibody

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0.2 mg / €690.00

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Mouse anti Human, Rabbit Filamin TI10

AM31463PU-N

Product Description for Filamin

Mouse anti Human, Rabbit Filamin TI10.
Properties: (90 kDa)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Filamin

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms FLNA, FN1, FN2, FN3, FNLB, FNLC
Presentation Purified
Reactivity Hu, Rb
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone TI10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Semi-pure Human Filamin preparation.
Property 90 kDa
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunobotting.
Immunofluorescence.
Immunohistochemistry on Paraffin Sections: Use 1-2 µg/ml on formalin fixed tissue. Citrate/boiling retrieval is recommended when using this antibody on formalin-fixed paraffin-embedded sections.
Positive Control: Skin.
Negative Control: Brain.
Background Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules. The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half.
Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the
other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.
Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle. Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization.
Concentration 0.1 mg/ml (OD280nm)
General Readings
  1. Aakhus, A.M., et al., (1992) “Binding of human platelet glycoprotein Ib and actin to fragments of actin binding protein”, Thromb Haemost., 62(2):252-257.
Storage Store the antinbody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
PBS, pH 7.2 containing 0.09% Sodium Azide and 1% BSA
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
Recognizes Human and Rabbit Filamin (90 kDa epitope).
Immunoblots with the Filamin band (250kDa) from platelet,
lymphocyte, fibroblast and smooth muscle preparations. This epitope is located in the C-terminal of the 90kDa fragment produced by calpain cleavage.
Immunofluorescence staining on methanol fixed fibroblasts
shows strong stress fiber reactivity. Staining is cytoplasmic.
Species:
Rabbit, Human.

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