BM426P Filamin (250 kDa) antibody

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Mouse anti Chicken, Guinea Pig, Human, Rabbit, Rat Filamin PM6/317


Product Description for Filamin

Mouse anti Chicken, Guinea Pig, Human, Rabbit, Rat Filamin PM6/317.
Properties: (250 kDa)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Filamin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms FLNA, FN1, FN2, FN3, FNLB, FNLC
Presentation Purified
Reactivity Chk, GP, Hu, Rb, Rt
Applications P, WB
Clonality Monoclonal
Clone PM6/317
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Platelet Filamin.
Source: Tissue culture supernatant.
Property 250 kDa
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blot.
Immunohistochemistry on Formalin Fixed Paraffin Sections.
Background Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules. The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half.
Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the
other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.
Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle. Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization.
Concentration 0.1 mg/ml
General Readings
  1. Umeda T, Kouchi Z, Kawahara H, Tomioka S, Sasagawa N, Maeda T, et al. Limited proteolysis of filamin is catalyzed by caspase-3 in U937 and Jurkat cells. J Biochem. 2001 Oct;130(4):535-42. PubMed PMID: 11574073.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A Chromatography
Buffer System:
PBS containing 10mM Sodium Azide and 1mg/ml BSA
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
Human, Rabbit, Guinea Pig, Chicken and Rat.
Recognizes the Filamin band (250kDa) from platelets, lymphocytes, fibroblasts and smooth muscle.

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