discontinued

BM426S Filamin antibody

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2 ml / €450.00

Quick Overview

Mouse anti Guinea Pig, Human, Porcine Filamin PM6/317

BM426S

Product Description for Filamin

Mouse anti Guinea Pig, Human, Porcine Filamin PM6/317.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Filamin

Product Category Primary Antibodies
Quantity 2 ml
Synonyms FLNA, FN1, FN2, FN3, FNLB, FNLC
Presentation Supernatant
Reactivity GP, Hu, Por
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone PM6/317
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Human platelet plasma membrane. 
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Application Western Blotting.
Immunofluorescence.
Flow Cytometry: Use 10 µl of 1/5 diluted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. 
Recommended Positive Control: Smooth muscle.
General Readings
  1. Aakhus AM, Wilkinson M, Pedersen TM, Solum NO. The use of PhastSystem crossed immunoelectrophoresis with immunoblotting to demonstrate a complex between glycoprotein Ib and the actin-binding protein (ABP) of human platelets. Electrophoresis. 1989 Nov;10(11):758-61. PubMed PMID: 2612476.
  2. Xu Y, Bismar TA, Su J, Xu B, Kristiansen G, Varga Z, et al. Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion. J Exp Med. 2010 Oct 25;207(11):2421-37. doi: 10.1084/jem.20100433. Epub 2010 Oct 11. PubMed PMID: 20937704. (Free PMC Article available, 10 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Tissue Culture Supernatant
Supernatant
Specificity
Specificity:
This antibody recognizes both purified Filamin, and a 250kDa protein in cell lysates.
Clone PM6/317 has also been reported to work on Western blotting.
Species:
Human, Guinea Pig, Pig.

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