BM426S Filamin antibody

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Mouse anti Guinea Pig, Human, Porcine Filamin PM6/317


Product Description for Filamin

Mouse anti Guinea Pig, Human, Porcine Filamin PM6/317.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Filamin

Product Category Primary Antibodies
Quantity 2 ml
Synonyms FLNA, FN1, FN2, FN3, FNLB, FNLC
Presentation Supernatant
Reactivity GP, Hu, Por
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone PM6/317
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified Human platelet plasma membrane. 
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Application Western Blotting.
Flow Cytometry: Use 10 µl of 1/5 diluted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. 
Recommended Positive Control: Smooth muscle.
General Readings
  1. Aakhus AM, Wilkinson M, Pedersen TM, Solum NO. The use of PhastSystem crossed immunoelectrophoresis with immunoblotting to demonstrate a complex between glycoprotein Ib and the actin-binding protein (ABP) of human platelets. Electrophoresis. 1989 Nov;10(11):758-61. PubMed PMID: 2612476.
  2. Xu Y, Bismar TA, Su J, Xu B, Kristiansen G, Varga Z, et al. Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion. J Exp Med. 2010 Oct 25;207(11):2421-37. doi: 10.1084/jem.20100433. Epub 2010 Oct 11. PubMed PMID: 20937704. (Free PMC Article available, 10 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
0.09% Sodium Azide
Liquid Tissue Culture Supernatant
This antibody recognizes both purified Filamin, and a 250kDa protein in cell lysates.
Clone PM6/317 has also been reported to work on Western blotting.
Human, Guinea Pig, Pig.

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