AP09203BT-N FITC antibody

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1 mg / €580.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti FITC

AP09203BT-N

Product Description for FITC

Goat anti FITC.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for FITC

Product Category Primary Antibodies
Target Category
Quantity 1 mg
Synonyms Fluorescein Isothiocyanate
Presentation Biotin
Applications C, E, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Fluorescein conjugated to Goat IgG
Application ELISA: 1/115,000.
Western Blot: 1/1,000 - 1/3,000.
Immunohistochemistry.
Background Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Goat IgG molecule
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
State:
Lyophilized purified Ig
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage mix product with 50% glycerol.
Specificity
Specificity:
This antibody reacts to Fluorescein.

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