AP09428TR-N FITC F(ab)2 Fragment antibody

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1 mg / €550.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti FITC

AP09428TR-N

Product Description for FITC

Goat anti FITC.
Properties: F(ab)2 Fragment
Presentation: Texas Red
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for FITC

Product Category Primary Antibodies
Target Category
Quantity 1 mg
Synonyms Fluorescein Isothiocyanate
Presentation Texas Red
Applications F, ICC/IF
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Fluorescein conjugated to Goat IgG
Property F(ab)2 Fragment
Application Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow cytometry: 1:2,000 - 1:10,000.
Immunoflourescence microscopy: 1:500 - 1:2,500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings (Conjugation) J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, 1982.
Storage Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
Format
Label:
Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons)
Texas Red
Absorption emission:
596 nm / 620 nm
Molar radio:
3.5 moles Texas RedTM per mole of Goat IgG
Purification:
Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide
State:
Lyophilized Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody detects Fluorescein F(ab')2.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

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