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AM09071PU-N FKBP4 antibody

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0.1 ml / €310.00
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Mouse anti Human FKBP4 AT4D3

AM09071PU-N

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  • AM09071PU-N

Product Description for FKBP4

Mouse anti Human FKBP4 AT4D3.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for FKBP4

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms 52 kDa FK506-binding protein, FK506-binding protein 4, FKBP52 protein, FKBP59, HBI, HSP-binding immunophilin, PPIase, Peptidyl-prolyl cis-trans isomerase, Rotamase, p59 protein
Presentation Purified
Reactivity Hu
Applications E, P, WB
Clonality Monoclonal
Clone AT4D3
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02790
Immunogen:
Recombinant Human FKBP4 (1-459 aa) purified from E. coli
GeneID:
2288
Isotype control AM03110PU-N, SM12P
Application ELISA.
Western blot (1/1,000-1/2,000). 
Cell lysates of HeLa (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FkBP4 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. 
Immunohistochemistry on Paraffin Embedded Tissue (1/50-1/100). 
Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-Human FKBP4 (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Background FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Ott M, Litzenburger UM, Rauschenbach KJ, Bunse L, Ochs K, Sahm F, et al. Suppression of TDO-mediated tryptophan catabolism in glioblastoma cells by a steroid-responsive FKBP52-dependent pathway. Glia. 2015 Jan;63(1):78-90. doi: 10.1002/glia.22734. Epub 2014 Aug 5. PubMed PMID: 25132599.
General Readings
  1. Ostrow KL, Park HL, Hoque MO, Kim MS, Liu J, Argani P, et al. Pharmacologic unmasking of epigenetically silenced genes in breast cancer. Clin Cancer Res. 2009 Feb 15;15(4):1184-91. doi: 10.1158/1078-0432.CCR-08-1304. PubMed PMID: 19228724. (Free PMC Article available, 2 images available)
  2. Ma D, Bai X, Guo S, Jiang Y. The switch I region of Rheb is critical for its interaction with FKBP38. J Biol Chem. 2008 Sep 19;283(38):25963-70. doi: 10.1074/jbc.M802356200. Epub 2008 Jul 25. PubMed PMID: 18658153. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
The antibody recognizes Human FKBP4. Other species not tested.
Gene ID 2288

Accessory Products

Proteins and/or Positive Controls

Proteins for FKBP4 (9 products)

Catalog No. Species Pres. Purity   Source  

FKBP4
FKBP4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FKBP4 (1-459, His-tag)
FKBP4, 1-459aa human, His-tagged, recombinant, E.coli: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  OriGene Technologies GmbH

FKBP4 (1-459, His-tag)
FKBP4, 1-459aa human, His-tagged, recombinant, E.coli: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €230.00
  OriGene Technologies GmbH

FKBP4
FKBP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FKBP4
FKBP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FKBP4
FKBP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FKBP4
FKBP4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FKBP4
FKBP4 Human
  Abnova Taiwan Corp.

FKBP4
SDS-Page: FKBP4/FKBP52 Protein [NBC1-22936] - FKBP4, 53.9 kDa (479 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for FKBP4 (3 products)

Catalog No. Species Pres. Purity   Source  

FKBP4 293T Cell Transient Overexpression Lysate(Denatured)
FKBP4 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

FKBP4/FKBP52 Lysate
Western Blot: FKBP4/FKBP52 Lysate [NBL1-10738] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for FKBP4
  Novus Biologicals Inc.

FKBP4 overexpression lysate
FKBP4 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for FKBP4
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