NB100-78498 Flagellin antibody

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Mouse anti Bacteria Flagellin FLIC-1

Product Description for Flagellin

Mouse anti Bacteria Flagellin FLIC-1.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Flagellin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms JW1908, b1923, flaF, fliC, hag
Presentation Purified
Reactivity Bac
Applications WB
Clonality Monoclonal
Clone FLIC-1
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
Isotype control AM03095PU-N
Application Western Blot, Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application., Western Blot
Background The FLIC-1 monoclonal antibody recognizes the flagellin subunit component FliC. FliC is a 51-44 kD protein expressed in many motile enteric bacteria including Salmonella and Escherichia. FliC is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers become polymerized to form the tail filament. The C-terminus of FliC binds to the export chaperone FliS. This interaction is thought to facilitate FliC polymerization and prevent FliC from polymerizing prematurely in cytosol.
Concentration 0.5 mg/ml
General Readings
  1. Masten, B.J., and Joys, T.M. 1993. J. Bacteriol. 175:5359.2. Kuwajima, G., et al. 1986. J. Bacteriol. 168:1479.3. Evdokimov, A.G., et al. 2003. Nature Struct. Biol. 10:789.
Storage Store at 4C. Do not freeze.
protein G purified
Buffer System:
0.09% Sodium Azide
protein G purified
FliC (FLIC-1)
Gene ID 949101

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