SP6239P FLASH (C-term) antibody

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Rabbit anti Human, Mouse FLASH (C-term)


Product Description for FLASH (C-term)

Rabbit anti Human, Mouse FLASH (C-term).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for FLASH (C-term)

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

Rabbit anti-FLASH (CT) polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid (1, 2).
Application Western blot (0.25 - .05 µg/ml). HeLa cell lysate can be used as positive control and an approximate 220 kDa band can be detected. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Imai Y, Kimura T, Murakami A, Yajima N, Sakamaki K, Yonehara S. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature. 1999 Apr 29;398(6730):777-85. PubMed PMID: 10235259.
  2. Koonin EV, Aravind L, Hofmann K, Tschopp J, Dixit VM Apoptosis. Searching for FLASH domains. Nature 1999; 401:662-3.
  3. Medema JP. Apoptosis. Life and death in
Storage Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Aff - Purified
This antibody recognises the C-terminal region of FLICE - associated huge protein (FLASH).
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1, 2). FLASH is widely expressed.

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