discontinued

AM10167SU-N Fluorescein (FITC) antibody

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Search for all "Fluorescein (FITC)"

0.2 ml / €350.00

Quick Overview

Mouse anti Fluorescein (FITC) 6A4

AM10167SU-N

Product Description for Fluorescein (FITC)

Mouse anti Fluorescein (FITC) 6A4.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Fluorescein (FITC)

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Ascites
Applications C, E, WB
Clonality Monoclonal
Clone 6A4
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Fluorescein (FITC)
Application

ELISA: 1/2000-1/20000.
Western Blot: 1/200-1/2000.
Immunohistochemistry: 1/500-1/5000.

Background Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
Storage

Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: Six months from despatch.

Format
Preservatives:
None
State:
Lyophilized powder
Ascites
Reconstitution:
Restore in distilled water.
Specificity
Specificity:
Reacts with free and bound Fluorescein

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