AP02423PU-N FOXO1A pSer319 antibody

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0.1 mg / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat FOXO1A

AP02423PU-N

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Product Description for FOXO1A

Rabbit anti Human, Mouse, Rat FOXO1A.
Properties: pSer319
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for FOXO1A

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q12778
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of Serine 319 (T-S-SP-N-A).
GeneID:
2308
Property pSer319
Add. information All Sites:
Human: Ser319
Mouse: Ser316
Rat: Ser313
Application Western Blot: 1/500-1/1000.
Immunofluorescence: 1/100-1/200.
Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Background FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.
Concentration 1.0 mg/ml
General Readings
  1. Rena G, et al. (2002) EMBO J 21(9): 2263-2271.
  2. Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607.
  3. Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Cchromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of FKHR only when phosphorylated at Serine 319.
Species:
Human, Rat and Mouse.
Gene ID 2308
FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for FOXO1A (3 products)

Catalog No. Species Pres. Purity   Source  

FOXO1A

FOXO1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

FOXO1A

FOXO1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FOXO1A

FOXO1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for FOXO1A (2 products)

Catalog No. Species Pres. Purity   Source  

FOXO1A Lysate

Western Blot: FOXO1A Lysate [NBL1-10813] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for FOXO1
  Novus Biologicals Inc.

FOXO1 overexpression lysate

FOXO1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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