AP02666PU-S FOXO1A (254-258) antibody

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50 µg / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human FOXO1A

AP02666PU-S

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  • AP02666PU-S
  • AP02666PU-S

Product Description for FOXO1A

Rabbit anti Human FOXO1A.
Properties: (254-258)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for FOXO1A

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 78-82 KDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q12778
Immunogen:
Peptide Sequence around amino acids 254~258 (A-A-S-M-D) derived from Human FKHR/FOXO1A
GeneID:
2308
Property 254-258
Application Western blot: 1/500-1/1000.
Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1%Tween-20 at 4°C with gentle shaking, obernight.
Immunofluorescence: 1/100-1/200.
Immunohistochemistry on Paraffin sections: 1/50-1/100.
Background FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.
Concentration 1.0 mg/ml
General Readings
  1. Gan L, et al. (2005) J Neurochem; 93(5): 1209-19.
  2. Smith WW, et al. (2005) J Cell Biol; 169(2): 331-9.
  3. Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77.
  4. Horn S, et al. (2004) Leukemia; 18(11): 1839-49.
  5. Zhao X, et al. (2004) Biochem J 4; 378(Pt 3): 839-49.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Immunoaffinity Chromatography using epitope-specific immunogen.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human tissue and cell lines
Specificity
Specificity:
This antibody detects endogenous levels of total FKHR protein.
Gene ID 2308
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for FOXO1A (3 products)

Catalog No. Species Pres. Purity   Source  

FOXO1A

FOXO1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FOXO1A

FOXO1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

FOXO1A

FOXO1A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for FOXO1A (2 products)

Catalog No. Species Pres. Purity   Source  

FOXO1A Lysate

Western Blot: FOXO1A Lysate [NBL1-10813] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for FOXO1
  Novus Biologicals Inc.

FOXO1 overexpression lysate

FOXO1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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