R1081 Fructose 6 Phosphate Kinase antibody

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2 ml / €1,180.00
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Goat anti Rabbit Fructose 6 Phosphate Kinase


Product Description for Fructose 6 Phosphate Kinase

Goat anti Rabbit Fructose 6 Phosphate Kinase.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Fructose 6 Phosphate Kinase

Product Category Primary Antibodies
Quantity 2 ml
Presentation Serum
Reactivity Rb
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Fructose-6-Phosphate Kinase from Rabbit Muscle.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation.
Recommended Dilutions: This product has been assayed against 1.0 µg of Fructose-6-Phosphate Kinase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Background Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme.
Concentration ~90.0 mg/ml (by Refractometry)
Storage Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot reconstituted contents and freeze at -20°C or below.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Prepared from monospecific antiserum by delipidation and defibrination.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide as preservative.
Lyophilized purified Ig fraction.
Restore with 2.0 ml of deionized water (or equivalent).
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].
Cross reactivity may occur against other mammalian Fructose-6-Phosphate Kinase, but were not specifically determined.

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