R1081B Fructose 6 Phosphate Kinase antibody

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Goat anti Rabbit Fructose 6 Phosphate Kinase


Product Description for Fructose 6 Phosphate Kinase

Goat anti Rabbit Fructose 6 Phosphate Kinase.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Fructose 6 Phosphate Kinase

Product Category Primary Antibodies
Quantity 10 mg
Presentation Biotin
Reactivity Rb
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Fructose-6-Phosphate Kinase [Rabbit muscle].
Application Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:6,000 to 1:28,000 of the reconstitution concentration is suggested for this product.
Background Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
General Readings Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20°C or below.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio:
10-20 BAC molecules per Goat IgG molecule.
Multi-step process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Lyophilized purified Ig fraction.
Restore with 1.0 ml of deionized water (or equivalent).
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].
Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.

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