R1147P Fructosyl-Amino Acid Oxidase [E.coli] antibody

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Sheep anti Fructosyl-Amino Acid Oxidase [E.coli]


Product Description for Fructosyl-Amino Acid Oxidase [E.coli]

Sheep anti Fructosyl-Amino Acid Oxidase [E.coli].
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Fructosyl-Amino Acid Oxidase [E.coli]

Product Category Primary Antibodies
Quantity 50 mg
Presentation Ig Fraction
Applications E, IP, WB
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Fructosyl-Amino Acid Oxidase [E.coli].
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods requiring high titer and specificity.
Recommended Diutions: This product has been assayed against 1.0 µg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Background Fructosyl amino acid oxidase (FAOX or FAOD) catalyzes the oxidative deglycation of fructosyl amino acids, yielding the corresponding amino acid, glucosone, and hydrogen peroxide. Glucose can be attached to aminoacids by a nonenzymatic reaction called "glycation", to distinguish it from the enzymatic glycosylation of proteins.
(FAOX) can be used for the enzymatic detection of nonenzymatically glycated proteins. Glycation of protein has been implicated in the development of diabetic complications and the aging process. Glycation of blood proteins such as hemoglobin and albumin is enhanced in diabetics with high blood glucose. The amounts of these glycated proteins reflect the level of blood glucose in periods corresponding to the half life of the protein (14 to 20 days for albumin and 1 to 2 months for hemoglobin). Since the glycation of blood proteins is not affected by transient increases in blood glucose, the levels of glycated proteins are good indices for monitoring diabetes mellitus patients during therapy. HbA1c, blood levels of which are a good index of the long term control of blood glucose in patients with diabetes mellitus, is defined as an amino end of the ß subunit (valine residue) of hemoglobin that has been glycated nonenzymatically.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Multi-step process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Lyophilized purified Ig fraction.
Ig Fraction
Restore with 5.0 ml of deionized water (or equivalent).
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.coli].
Cross reactivity against Fructosyl-Amino Acid Oxidase from other sources is unknown.

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