R1147HRPS Fructosyl-Amino Acid Oxidase antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Sheep anti Bacteria Fructosyl-Amino Acid Oxidase


Product Description for Fructosyl-Amino Acid Oxidase

Sheep anti Bacteria Fructosyl-Amino Acid Oxidase.
Presentation: HRP
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Enzyme Immunoassay.

Properties for Fructosyl-Amino Acid Oxidase

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation HRP
Reactivity Bac
Applications E, IP, WB
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Fructosyl-amino acid oxidase from Corynebacterium sp. (expressed in E.coli)
Application Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Recommended Diutions:
Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA: 1/5,000-1/20,000.
This product has been assayed against 1.0 µg of fructosyl-amino acid oxidase [Corynebacterium sp.] in a standard sandwich ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested for this product.
Background Fructosyl amino acid oxidase (FAOX or FAOD) catalyzes the oxidative deglycation of fructosyl amino acids, yielding the corresponding amino acid, glucosone, and hydrogen peroxide. Glucose can be attached to aminoacids by a nonenzymatic reaction called "glycation", to distinguish it from the enzymatic glycosylation of proteins.
FAOX can be used for the enzymatic detection of nonenzymatically glycated proteins. Glycation of protein has been implicated in the development of diabetic complications and the aging process. Glycation of blood proteins such as hemoglobin and albumin is enhanced in diabetics with high blood glucose. The amounts of these glycated proteins reflect the level of blood glucose in periods corresponding to the half life of the protein (14 to 20 days for albumin and 1 to 2 months for hemoglobin). Since the glycation of blood proteins is not affected by transient increases in blood glucose, the levels of glycated proteins are good indices for monitoring diabetes mellitus patients during therapy. HbA1c, blood levels of which are a good index of the long term control of blood glucose in patients with diabetes mellitus, is defined as an amino end of the ß subunit (valine residue) of hemoglobin that has been glycated nonenzymatically.
Caution Do NOT add Sodium azide!
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish peroxidase
Multi-step process  includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
10 mg/ml BSA (immunoglobulin and protease free)
Lyophilized purified Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This antibody detects fructosyl-amino acid oxidase [Corynebacterium sp.].
Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified fructosyl-amino acid oxidase [Coryneb. sp.].
Corynebacterium sp.

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