NB100-75150 Gag polyprotein antibody

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Chicken anti Gag polyprotein

Product Description for Gag polyprotein

Chicken anti Gag polyprotein.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Gag polyprotein

Product Category Primary Antibodies
Quantity 0.05 mg
Synonyms GAG, Gag polyprotein, Pr55Gag
Presentation Aff - Purified
Applications WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The immunogen for this antibody was a sequence from 2a.a. to 132a.a. from Gag polyprotein(a 500a.a. long protein)
Application Western Blot, Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development., Western Blot
Background Matrix protein p17 targets Gag and Gag-Pol polyproteinss to the plasma membrane via a multipartite membrane binding signal. that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.
General Readings
  1. Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J., et al.. Complete Plasmid Sequence of pNL4-3
  2. Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J., et al.. Direct Submission
  3. , Buckler C.E., Buckler-White A.J., Willey R.L., McCoy J.. Submitted (JUN-1988) to the EMBL/GenBank/DDBJ databases.
  4. , VerPlank L., Bouamr F., LaGrassa T.J., Agresta B., Kikonyogo A., Leis J., Carter C.A.. Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag).
  5. , Garrus J.E., von Schwedler U.K., Pornillos O.W., Morham S.G., Zavitz K.H., Wang H.E., Wettstein D.A., Stray K.M., Cote M., Rich R.L., et al.. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
  6. , Scarlata S., Carter C.. Role of HIV-1 Gag domains in viral assembly
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
No Preservative
Immunogen affinity purified
Aff - Purified
Gag polyprotein

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