AM10173SU-N Ganglioside GD3 antibody

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1 ml / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Ganglioside GD3 4F6

AM10173SU-N

Product Description for Ganglioside GD3

Mouse anti Ganglioside GD3 4F6.
Presentation: Supernatant
Product is tested for Enzyme Immunoassay.

Properties for Ganglioside GD3

Product Category Primary Antibodies
Quantity 1 ml
Presentation Supernatant
Applications E
Clonality Monoclonal
Clone 4F6
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Ganglioside-GD3
Application

ELISA: 1/5-1/50.
Thin-layer chromatography (TLC): 1/5-1/20.

Background Almost all melanomas, astrocytomas, a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen. It is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in Humans via enhancement of cytotoxic and proliferative response of lymphocytes.
General Readings
  1. Natunen S. et al. Novel acidic glycan markers of human cells. Patent Application publication. 2011 feb 24.
  2. Kang NY, Kang Y, Kang SK, Lee YC, Choi HJ, Lee YS, et al. Transcriptional regulation of the human GD3 synthase gene expression in Fas-induced Jurkat T cells: a critical role of transcription factor NF-kappaB in regulated expression. Glycobiology. 2006 May;16(5):375-89. Epub 2006 Feb 15. PubMed PMID: 16481330.
  3. Cerato E, Birkle S, Portoukalian J, Mezazigh A, Chatal JF, Aubry J. Variable region gene segments of nine monoclonal antibodies specific to disialogangliosides (GD2, GD3) and their O-acetylated derivatives. Hybridoma. 1997 Aug;16(4):307-16. PubMed PMID: 9309421.
  4. Thomas CP, Buronfosse A, Combaret V, Pedron S, Fertil B, Portoukalian J. Gangliosides protect human melanoma cells from ionizing radiation-induced clonogenic cell death. Glycoconj J. 1996 Jun;13(3):377-84. PubMed PMID: 8781968.
  5. Zebda N, Pedron S, Rebbaa A, Portoukalian J, Berthier-Vergnes O. Deficiency of ganglioside biosynthesis in metastatic human melanoma cells: relevance of CMP-NeuAc:LacCer alpha 2-3 sialyltransferase (GM3 synthase). FEBS Lett. 1995 Apr 3;362(2):161-4. PubMed PMID: 7720864.
  6. Portoukalian J, David MJ, Gain P, Richard M. Shedding of GD2 ganglioside in patients with retinoblastoma. Int J Cancer. 1993 Apr 1;53(6):948-51. PubMed PMID: 8473052.
Storage Store prior to reconstitution at -20°C. 
Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
None
State:
Lyophilized Cell Culture Supernatant
Supernatant
Reconstitution:
Restore in distilled water.
Specificity
Specificity:
Reacts with GD3 Ganglioside: (NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1Cer).
Cross reacts with 9-O-AcGD3 ganglioside (9OAcNeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc beta 1-1Cer) and GT1a ganglioside (NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4GalNAc beta 1-4 (NeuAc alpha 2-3) Gal beta 1-4Glc beta 1-1Cer).

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