ACR001P GAPDH (Loading Control) antibody

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0.2 mg / €270.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Canine, Feline, Fish, Human, Mouse, Porcine, Rabbit, Rat GAPDH 6C5

ACR001P

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Product Description for GAPDH

Mouse anti Canine, Feline, Fish, Human, Mouse, Porcine, Rabbit, Rat GAPDH 6C5.
Properties: (Loading Control)
Presentation: Purified
Product is tested for Immunoprecipitation, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for GAPDH

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
Presentation Purified
Reactivity Can, Fe, Fi, Hu, Ms, Por, Rb, Rt
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone 6C5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Manufacturer certificates ISO 9001:2008

Datasheet Extract

Immunogen
Swiss Prot Num:
P04406
Immunogen:
Rabbit GAPDH.
GeneID:
2597
Remarks:
Hybridoma is derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
Property Loading Control
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application GAPDH Immunoassays.
Western blot (e.g. as Loading Control).
Immunocytochemistry.
Background Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required.
Concentration 1 mg/ml
Product Citations
Purchased from Acris:
  1. Kadow S, Jux B, Zahner SP, Wingerath B, Chmill S, Clausen BE, et al. Aryl hydrocarbon receptor is critical for homeostasis of invariant gammadelta T cells in the murine epidermis. J Immunol. 2011 Sep 15;187(6):3104-10. doi: 10.4049/jimmunol.1100912. Epub 2011 Aug 15. PubMed PMID: 21844385.
  2. Jux B, Kadow S, Esser C. Langerhans cell maturation and contact hypersensitivity are impaired in aryl hydrocarbon receptor-null mice. J Immunol. 2009 Jun 1;182(11):6709-17. doi: 10.4049/jimmunol.0713344. PubMed PMID: 19454665.
  3. Salameh A, Krautblatter S, Baessler S, Karl S, Rojas Gomez D, Dhein S, et al. Signal transduction and transcriptional control of cardiac connexin43 up-regulation after alpha 1-adrenoceptor stimulation. J Pharmacol Exp Ther. 2008 Jul;326(1):315-22. doi: 10.1124/jpet.108.136663. Epub 2008 Apr 29. PubMed PMID: 18445782.
  4. Wachi T, Cornell B, Marshall C, Zhukarev V, Baas PW, Toyo-Oka K. Ablation of the 14-3-3gamma Protein Results in Neuronal Migration Delay and Morphological Defects in the Developing Cerebral Cortex. Dev Neurobiol. 2015 Aug 22. doi: 10.1002/dneu.22335. PubMed PMID: 26297819.
  5. Columbus DA, Steinhoff-Wagner J, Suryawan A, Nguyen HV, Hernandez-Garcia A, Fiorotto ML, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015 Sep 15;309(6):E601-10. doi: 10.1152/ajpendo.00089.2015. Epub 2015 Aug 4. PubMed PMID: 26374843. (Free PMC Article available)
General Readings
  1. Kots AYa, Skurat AV, Sergienko EA, Bulargina TV, Severin ES. Nitroprusside stimulates the cysteine-specific mono(ADP-ribosylation) of glyceraldehyde-3-phosphate dehydrogenase from human erythrocytes. FEBS Lett. 1992 Mar 23;300(1):9-12. PubMed PMID: 1547895.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A Sepharose Chromatography
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Porcine, Canine, Rabbit, Cat, Rat, Mouse and Fish.Does NOT react with Bovine and Goat.
Specificity
Specificity:
This antibody reacts with GAPDH.
Gene ID 2597
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for GAPDH (18 products)

Catalog No. Species Pres. Purity   Source  

GAPDH

GAPDH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GAPDH (1-335)

GAPDH Human Purified > 95 % pure by SDS-PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

GAPDH (1-335)

GAPDH Human Purified > 95 % pure by SDS-PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

GAPDH

GAPDH Human Liquid ≥ 95 % E. coli
20 µg / €400.00
  GeneTex Inc.

GAPDH

GAPDH Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human
0.5 mg / €330.00
  Abnova Taiwan Corp.

GAPDH

GAPDH Human Purified > 98 % Heart
1 mg / €920.00
  HyTest Ltd.

GAPDH

GAPDH Rabbit Purified > 98 % Heart
1 mg / €920.00
  HyTest Ltd.

GAPDH

SDS-Page: GAPDH Protein [NBC1-18528] - GAPDH, 36kDa (335aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
0.1 mg / €380.00
  Novus Biologicals Inc.

GAPDH

GAPDH Human Purified
0.2 mg / €320.00
  Novus Biologicals Inc.

GAPDH

GAPDH Human Purified
0.2 mg / €320.00
  Novus Biologicals Inc.

GAPDH

GAPDH Human Purified
0.2 mg / €320.00
  Novus Biologicals Inc.

Positive controls for GAPDH (2 products)

Catalog No. Species Pres. Purity   Source  

GAPDH Lysate(Denatured)

GAPDH Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

GAPDH Lysate

Western Blot: GAPDH Lysate [NBL1-10967] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for GAPDH
0.1 mg / €400.00
  Novus Biologicals Inc.
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