NB120-6217 GD3 antibody

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Mouse anti GD3 4F6


Product Description for GD3

Mouse anti GD3 4F6.
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for GD3

Product Category Primary Antibodies
Quantity 1 ml
Applications E, WB
Clonality Monoclonal
Clone 4F6
Host Mouse
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Reconstitute in 1 ml of distilled water.
Application ELISA, Western Blot, ELISA: Use at a dilution of 1/10 - 1/100. TLC: Use at an assay dependant dilution. WB: Use at a dilution of 1/10 - 1/100. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, Western Blot
Background Almost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
General Readings General / background references:Cerato E et al. Variable region gene segments of nine monoclonal antibodies specific to disialogangliosides (GD2, GD3) and their O-acetylated derivatives. Hybridoma 16:307-16 (1997). PubMed PMID: 9309421
Thomas CP et al. Gangliosides protect human melanoma cells from ionizing radiation-induced clonogenic cell death. Glycoconj J 13:377-84 (1996). PubMed PMID: 8781968
Zebda N et al. Deficiency of ganglioside biosynthesis in metastatic human melanoma cells: relevance of CMP-NeuAc:LacCer alpha 2-3 sialyltransferase (GM3 synthase). FEBS Lett 362:161-4 (1995). PubMed PMID: 7720864
Portoukalian J et al. Shedding of GD2 ganglioside in patients with retinoblastoma. Int J Cancer 53:948-51 (1993). PubMed PMID: 8473052
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tissue culture supernatant
Buffer System:
No Preservative
Tissue culture supernatant
The reacts specifically with GD3 ganglioside (NeuAc

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