SP3005P GFP antibody

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0.1 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti All Species GFP

SP3005P

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  • SP3005P

Product Description for GFP

Rabbit anti All Species GFP.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for GFP

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms GFP-Tag, Green fluorescent protein
Presentation Purified
Reactivity All Species
Applications ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P42212
Immunogen:
EGFP, a native full-length protein
Application Immunoprecipitation: 10-20 µg/sample. 
Immunocytochemistry: 1-3 µg/ml. 
Western blot: 0.5-1.5 µg/ml. 
Positive Control: transfected cells. 
Negative Control: non-transfected cells.
Background Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm).
GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.
GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Ling YH, Wong CC, Li KW, Chan KM, Boukamp P, Liu WK. CCHCR1 interacts with EDC4, suggesting its localization in P-bodies. Exp Cell Res. 2014 Sep 10;327(1):12-23. doi: 10.1016/j.yexcr.2014.05.008. Epub 2014 May 21. PubMed PMID: 24858563.
  2. Gentzel M, Schille C, Rauschenberger V, Schambony A. Distinct functionality of Dishevelled isoforms on Ca2+/Calmodulin dependent protein kinase 2 (CamKII) in Xenopus gastrulation. Mol Biol Cell. 2015 Jan 7. pii: mbc.E14-06-1089. PubMed PMID: 25568338.
Originator or purchased from resellers:
  1. Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V. HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. Epub 2006 May 25. PubMed PMID: 16724116. (Free PMC Article available, 10 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS
Preservatives:
15 mM Sodium Azide
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Purified
Specificity
Specificity:
The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.
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Accessory Products

Proteins and/or Positive Controls

Proteins for GFP (5 products)

Catalog No. Species Pres. Purity   Source  

GFP (1-238)

Prepare  150 µl recombinant GFP with various concentrations (0.00074 nM - 6.21 µM) inassay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide).
Read at extinction wavelength 485 nm and emission at 535 nm.

96 well Polystyrene microplate, black (Greiner Bio-One, Cat.No. 655077), Fluorescence plate reader (PerkinElmer, Victor X3) A. victoria Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €680.00
  Acris Antibodies GmbH

GFP (1-238)

Prepare  150 µl recombinant GFP with various concentrations (0.00074 nM - 6.21 µM) inassay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide).
Read at extinction wavelength 485 nm and emission at 535 nm.

96 well Polystyrene microplate, black (Greiner Bio-One, Cat.No. 655077), Fluorescence plate reader (PerkinElmer, Victor X3) A. victoria Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €260.00
  Acris Antibodies GmbH

GFP

GFP
0.1 mg / €380.00
  Abnova Taiwan Corp.

GFP

GFP A. victoria Purified E. coli
10 µg / €220.00
  Alpha Diagnostic Intl. Inc.

GFP

GFP A. victoria Purified E. coli
0.1 mg / €890.00
  Alpha Diagnostic Intl. Inc.
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