TP401 GFP antibody

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0.2 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti A. victoria GFP

TP401

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  • TP401

Product Description for GFP

Rabbit anti A. victoria GFP.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for GFP

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms GFP-Tag, Green fluorescent protein
Presentation Purified
Reactivity A. victoria
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Torrey Pines Biolabs

Datasheet Extract

Immunogen
Swiss Prot Num:
P42212
Immunogen:
E.coli expressed full-length GFP (Green Fluorescent Protein).
Add. information This product was originally produced by Torrey Pine Biolabs
Application ELISA.
Western blot: 1/5,000.
Immunoprecipitation: 1/200-1/500.
The antibody has successfully been used in Immunohistochemistry on Vibratome sections and in Filter Binding Assays.
Background Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA1 and demonstration that GFP can be expressed as a functional transgene2, GFP has become a new tool with exciting applications in developmental, cell and molecular biology3. GFP is an ideal fluorescent probe: its fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells; it can fuse with proteins of interest without interfering significantly with their assembly or function.
Product Citations
Purchased from Acris:
  1. Albert M, Jehle AK, Mueller K, Eisele C, Lipschis M, Felix G. Arabidopsis thaliana pattern recognition receptors for bacterial elongation factor Tu and flagellin can be combined to form functional chimeric receptors. J Biol Chem. 2010 Jun 18;285(25):19035-42. doi: 10.1074/jbc.M110.124800. Epub 2010 Apr 21. PubMed PMID: 20410299. (Free PMC Article available, 5 images available)
  2. Angenendt P, Kreutzberger J, Glökler J, Hoheisel JD. Generation of high density protein microarrays by cell-free in situ expression of unpurified PCR products. Mol Cell Proteomics. 2006 Sep;5(9):1658-66. Epub 2006 Jul 5. PubMed PMID: 16825183.
  3. Li N, Kelsh RN, Croucher P, Roehl HH. Regulation of neural crest cell fate by the retinoic acid and Pparg signalling pathways. Development. 2010 Feb;137(3):389-94. doi: 10.1242/dev.044164. PubMed PMID: 20081187. (Free PMC Article available, 4 images available)
  4. Poulin LF, Henri S, de Bovis B, Devilard E, Kissenpfennig A, Malissen B. The dermis contains langerin+ dendritic cells that develop and function independently of epidermal Langerhans cells. J Exp Med. 2007 Dec 24;204(13):3119-31. Epub 2007 Dec 17. PubMed PMID: 18086861. (Free PMC Article available, 7 images available)
  5. Yusuf F, Rehimi R, Moroşan-Puopolo G, Dai F, Zhang X, Brand-Saberi B. Inhibitors of CXCR4 affect the migration and fate of CXCR4+ progenitors in the developing limb of chick embryos. Dev Dyn. 2006 Nov;235(11):3007-15. PubMed PMID: 16958136.
  6. Blachon S, Bellanger S, Demeret C, Thierry F. Nucleo-cytoplasmic shuttling of high risk human Papillomavirus E2 proteins induces apoptosis. J Biol Chem. 2005 Oct 28;280(43):36088-98. Epub 2005 Aug 31. PubMed PMID: 16135518.
  7. Sampaio KL, Cavignac Y, Stierhof YD, Sinzger C. Human cytomegalovirus labeled with green fluorescent protein for live analysis of intracellular particle movements. J Virol. 2005 Mar;79(5):2754-67. PubMed PMID: 15708994. (Free PMC Article available, 5 images available)
  8. Rynes J, Donohoe CD, Frommolt P, Brodesser S, Jindra M, Uhlirova M. Activating transcription factor 3 regulates immune and metabolic homeostasis. Mol Cell Biol. 2012 Oct;32(19):3949-62. doi: 10.1128/MCB.00429-12. Epub 2012 Jul 30. PubMed PMID: 22851689. (Free PMC Article available, 9 images available)
General Readings
  1. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 1992 Feb 15;111(2):229-33. PubMed PMID: 1347277.
  2. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science. 1994 Feb 11;263(5148):802-5. PubMed PMID: 8303295.
  3. Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-44. PubMed PMID: 9759496.
Storage Store the antibody undiluted at 2-8°C for one month
or (in small aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch
Format
Purification:
Protein A Chromatography
Preservatives:
0.09% Sodium Azide
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore to 1 mg/ml by adding 0.2 ml water.
Specificity
Specificity:
This antibody reacts with wild-type GFP, and its variants such as EGFP and EBFP.
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for GFP (5 products)

Catalog No. Species Pres. Purity   Source  

GFP (1-238)

Prepare  150 µl recombinant GFP with various concentrations (0.00074 nM - 6.21 µM) inassay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide).
Read at extinction wavelength 485 nm and emission at 535 nm.

96 well Polystyrene microplate, black (Greiner Bio-One, Cat.No. 655077), Fluorescence plate reader (PerkinElmer, Victor X3) A. victoria Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €680.00
  Acris Antibodies GmbH

GFP (1-238)

Prepare  150 µl recombinant GFP with various concentrations (0.00074 nM - 6.21 µM) inassay buffer and equilibrate to 25°C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide).
Read at extinction wavelength 485 nm and emission at 535 nm.

96 well Polystyrene microplate, black (Greiner Bio-One, Cat.No. 655077), Fluorescence plate reader (PerkinElmer, Victor X3) A. victoria Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €260.00
  Acris Antibodies GmbH

GFP

GFP
0.1 mg / €380.00
  Abnova Taiwan Corp.

GFP

GFP A. victoria Purified E. coli
10 µg / €220.00
  Alpha Diagnostic Intl. Inc.

GFP

GFP A. victoria Purified E. coli
0.1 mg / €890.00
  Alpha Diagnostic Intl. Inc.
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