NBP1-07021 GFPuv antibody

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Goat anti Human GFPuv


Product Description for GFPuv

Goat anti Human GFPuv.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for GFPuv

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Anti-GFP antibody, anti-Green Fluorescent Protein antibody
Presentation Aff - Purified
Reactivity Hu
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

E. coli-derived GFPuv

Produced in goats immunized with purified, E. coli-derived, recombinant GFPuv (aa 2 - 238; Accession # AAB65663). GFPuv specific IgG was purified by GFPuv affinity chromatography.
Application ELISA, immunoprecipitation, Western Blot, Western blot - The detection limit for GFPuv is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation - This antibody has been used to immunoprecipitate GFPuv from conditioned media of transfected NS0 cells. Direct ELISA - The detection limit for GFPuv is approximately 0.3 ng/well., ELISA 0.5 - 1.0 ug/mL, immunoprecipitation , Western Blot 0.1 - 0.2 ug/mL
Background Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490 - 520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a β-barrel with a chromophore (aa 65 - 67) containing α-helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.
Concentration 0.2 mg/ml
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Affinity purified
Buffer System:
No Preservative
Affinity purified
Aff - Purified

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