AP21370AF-N Glucose-6-phosphate isomerase (PGI1) antibody

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10 mg / €230.00
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Rabbit anti Bakers Yeast Glucose-6-phosphate isomerase (PGI1)


Product Description for Glucose-6-phosphate isomerase (PGI1)

Rabbit anti Bakers Yeast Glucose-6-phosphate isomerase (PGI1).
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Glucose-6-phosphate isomerase (PGI1)

Product Category Primary Antibodies
Quantity 10 mg
Synonyms NLK, Neuroleukin, PGI1, PHI, Phosphoglucose isomerase, Phosphohexose isomerase
Presentation Azide Free
Reactivity Bakers Yeast
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Phosphoglucose isomerase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.
Recommended working dilutions: vary widely, but may be between 1/1000 and 1/5000.
Concentration 10,0 mg/ml
Product Citations
Purchased from Acris:
  1. Miura N, Kirino A, Endo S, Morisaka H, Kuroda K, Takagi M, et al. Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion. Eukaryot Cell. 2012 Aug;11(8):1075-82. doi: 10.1128/EC.00075-12. Epub 2012 Jun 29. PubMed PMID: 22753847. (Free PMC Article available, 5 images available)
  2. Miura N, Ueda M. Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae. Methods Mol Biol. 2015;1270:51-70. doi: 10.1007/978-1-4939-2309-0_4. PubMed PMID: 25702108.
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins
Lyophilized hyperimmune IgG fraction
Azide Free
Restore by adding 1.0 ml of sterile distilled water
IgG fraction of polyclonal rabbit antiserum to Phosphoglucose Isomerase from Baker’s Yeast.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Gene ID 852495

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