NB500-117 Glutamyl Lysine gamma (Isopeptide) antibody

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Mouse anti All Species Glutamyl Lysine gamma (Isopeptide) 81D1C2


Product Description for Glutamyl Lysine gamma (Isopeptide)

Mouse anti All Species Glutamyl Lysine gamma (Isopeptide) 81D1C2.
Presentation: Ascites
Product is tested for ChIP assay, Western blot / Immunoblot.

Properties for Glutamyl Lysine gamma (Isopeptide)

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Ascites
Reactivity All Species
Applications ChIP, WB
Clonality Monoclonal
Clone 81D1C2
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic isopeptide.
Add. information Reconstitute in 200 ul of distilled water.
Application chromatin immunoprecipitation, immunohistochemistry, Western Blot, ChIP: Use 4-5ug for 106 cells.IHC: Use at a dilution of 1/50 - 1/800. WB: Use at a dilution of 1/50 - 1/500. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., chromatin immunoprecipitation, immunohistochemistry, Western Blot
General Readings This product has been used in:Johnson GV & LeShoure R Immunoblot analysis reveals that isopeptide antibodies do not specifically recognize the epsilon-(gamma-glutamyl)lysine bonds formed by transglutaminase activity. J Neurosci Methods 134:151-8 (2004). PubMed PMID: 15003381
Roch AM et al. Differential expression of isopeptide bonds N epsilon (gamma-glutamyl) lysine in benign and malignant human breast lesions: an immunohistochemical study. Int J Cancer 48:215-20 (1991). PubMed PMID: 1673451
This clone has been used in:el Alaoui S et al. Transglutaminase activity and N epsilon (gamma glutamyl) lysine isopeptide levels during cell growth: an enzymic and immunological study. Int J Cancer 48:221-6 (1991). PubMed PMID: 1673452
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
No Preservative
This reacts with the isopeptide : N
All Species

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