discontinued

AM32660PU-N Glutathione NEM antibody

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0.1 mg / €410.00

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Mouse anti Mammalia Glutathione 8.1GSH

AM32660PU-N

Product Description for Glutathione

Mouse anti Mammalia Glutathione 8.1GSH.
Properties: NEM
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Enzyme Immunoassay, Western blot / Immunoblot, Frozen Sections.

Properties for Glutathione

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Mam
Applications C, E, F, ICC/IF, WB
Clonality Monoclonal
Clone 8.1GSH
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
GS-NEM conjugated to KLH
Property NEM
Isotype control AM03095PU-N
Application ELISA: 1/100,000-1/200,000 to quantify GSH at ≥ 2 mM in biological specimens.
Flow Cytometry: 1/100-1/1,000
Immunocytochemistry: 1/100-1/500 for assessment of intracellular GSH distribution.
Immunohistochemistry: 1/100 (paraformaldehyde fixation).
Western Blotting.
Note: For use in Flow Cytometry or ELISA, sample should be first reacted with 10 μM NEM for 10 min at -70°C.
Concentration 1.0 mg/ml
General Readings
  1. Free Radical Biol. Med.(1995)18:709.
  2. Anticarcinogenesis and Radiation Protection. Cerutti, P., O. Nygaard, and M. Simic (Eds) (1987) pp. 407-412.
Storage

Store undiluted at 2-8°C.
Shelf life: one year from despatch.

Format
Purification:
Protein A Sepharose Chromatography
Buffer System:
0.02M PBS pH 7.6, 0.25M NaCl
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Mammals.
Specificity
Specificity:
Reactive with thiol-modified glutatione. Does not react with Oxidized Glutathione (GSSG) or Glutathione (GSH) in mixed disulfide linkage with proteins. AM32660PU-N is specific to the glutathione: N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide.

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