AM32660PU-N Glutathione NEM antibody

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Mouse anti Mammalia Glutathione 8.1GSH


Product Description for Glutathione

Mouse anti Mammalia Glutathione 8.1GSH.
Properties: NEM
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Enzyme Immunoassay, Western blot / Immunoblot, Frozen Sections.

Properties for Glutathione

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Mam
Applications C, E, F, ICC/IF, WB
Clonality Monoclonal
Clone 8.1GSH
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

GS-NEM conjugated to KLH
Property NEM
Isotype control AM03095PU-N
Application ELISA: 1/100,000-1/200,000 to quantify GSH at ≥ 2 mM in biological specimens.
Flow Cytometry: 1/100-1/1,000
Immunocytochemistry: 1/100-1/500 for assessment of intracellular GSH distribution.
Immunohistochemistry: 1/100 (paraformaldehyde fixation).
Western Blotting.
Note: For use in Flow Cytometry or ELISA, sample should be first reacted with 10 μM NEM for 10 min at -70°C.
Concentration 1.0 mg/ml
General Readings
  1. Free Radical Biol. Med.(1995)18:709.
  2. Anticarcinogenesis and Radiation Protection. Cerutti, P., O. Nygaard, and M. Simic (Eds) (1987) pp. 407-412.

Store undiluted at 2-8°C.
Shelf life: one year from despatch.

Protein A Sepharose Chromatography
Buffer System:
0.02M PBS pH 7.6, 0.25M NaCl
0.09% Sodium Azide
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
Reactive with thiol-modified glutatione. Does not react with Oxidized Glutathione (GSSG) or Glutathione (GSH) in mixed disulfide linkage with proteins. AM32660PU-N is specific to the glutathione: N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide.

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