discontinued

AM26737PU-N Glycogen Synthase Kinase-3 (GSK3) antibody

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0.1 mg / €310.00

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Mouse anti Drosophila, Human, Porcine, Rat Glycogen Synthase Kinase-3 (GSK3) 4G-1E

Product Description for Glycogen Synthase Kinase-3 (GSK3)

Mouse anti Drosophila, Human, Porcine, Rat Glycogen Synthase Kinase-3 (GSK3) 4G-1E.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for Glycogen Synthase Kinase-3 (GSK3)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Dros, Hu, Por, Rt
Applications P
Clonality Monoclonal
Clone 4G-1E
Host Mouse
Isotype IgG1
Shipping to USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide conjugate corresponding to peptide from Drosophila
AA Sequence:
(C)203KQLLHGEPNVSYICSR219Y
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Add. information Antigen distribution: The antigen has been described in the testis, thymus, prostate and ovary, weak in the brain, lung and kidney.
Application Immunohistochemistry on paraffin sections: 5 µg/ml (1/40). Microwave pretreatment in citrate buffer is recommended for antigen retrieval.Suggested positive control: Human brain.
Background GSK-3 has been highly conserved during evolution, and homolog genes have been identified in virtually every eukaryotic genome investigated. In mamm als, GSK-3 is encoded by two genes known as gsk-3 α and gsk-3 β , encoding GSK-3 α (483 aa in humans) and GSK - 3 β (433 aa) proteins with apparent molecular masses of 51 and 47kDa, respectively. Both isoforms are almost identical (98%) within their ATP binding pocket but differ in their N- and C-terminal sequences.
General Readings
  1. Harwood AJ, Plyte SE, Woodgett J, Strutt H, Kay RR. Glycogen synthase kinase 3 regulates cell fate in Dictyostelium. Cell. 1995 Jan 13;80(1):139-48. PubMed PMID: 7813009.
Storage Store lyophilized at 2-8°C for 6 month or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
Phosphate buffered saline pH 7.2 (PBS)
Preservatives:
0.01% Kathon
Stabilizers:
5mg/ml bovine serum albumin (BSA)
State:
Lyophilized affinity purified Ig fraction
Purified
Reconstitution:
Reconstitute by adding 0.5 ml distilled water. This stock solution co ntains 0.2 mg/ml IgG.
Species Reactivity
Species reactivity (tested):
Human, Rat, Porcine
Specificity
Specificity:
This antibody recognizes GSK-3 in most species. Monoclonal antibody 4G-1E was generated against the non-phosphorylated catalytic domain (between sub-domain VII and VIII) of the Drosophila GSK-3 / shaggy enzyme, corresponding to residues 268-284 in GSK-3 α and 205-221 in GSK-3 β, respectively.

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