AM26738PU-N Glycogen Synthase Kinase-3 (GSK3) (phospho specific) antibody

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0.1 mg / €330.00
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Mouse anti Human, Porcine, Rat Glycogen Synthase Kinase-3 (GSK3) 7G-1F2


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  • AM26738PU-N

Product Description for Glycogen Synthase Kinase-3 (GSK3)

Mouse anti Human, Porcine, Rat Glycogen Synthase Kinase-3 (GSK3) 7G-1F2.
Properties: (phospho specific)
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for Glycogen Synthase Kinase-3 (GSK3)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Por, Rt
Applications P
Clonality Monoclonal
Clone 7G-1F2
Host Mouse
Isotype IgG1
Shipping to USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Synthetic peptide conjugate corresponding to Drosophila
AA Sequence:
Property phospho specific
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Immunohistochemistry on paraffin sections: 1 µg/ml (1:200). Microwave pretreatment in citrate buffer is recommended for antigen retrieval.
Suggested positive control: Human brain.
Background GSK-3 has been highly conserved during evolution, and homolog genes have been identified in virtually every eukaryotic genome investigated. In mam mals, GSK-3 is encoded by two genes known as gsk-3 α and gsk-3β encoding GSK-3α (483 aa in humans) and GSK-3β (433 aa) proteins with apparent molecular masses of 51 and 47kDa, respectively. Both isoforms are almost identical (98%) within their ATP binding p ocket but differ in their N- and C- terminal sequences.
General Readings
  1. Harwood AJ, Plyte SE, Woodgett J, Strutt H, Kay RR. Glycogen synthase kinase 3 regulates cell fate in Dictyostelium. Cell. 1995 Jan 13;80(1):139-48. PubMed PMID: 7813009.
Storage Store lyophilized at 2-8°C for 6 month or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Phosphate buffered saline pH 7.2 (PBS)
0.01% Kathon
5 mg/ml bovine serum albumin (BSA)
Lyophilized affinity purified Ig fraction
Reconstitute by adding 0.5 ml distilled water. This stock solution contains 0.2 mg/ml IgG.
Species Reactivity
Species reactivity (tested):
Human, Rat, Porcine
This antibody recognizes GSK-3 in most species.
The antibody was generated against the phosphorylated catalytic domain (between sub-domain VII and VIII) of the Drosophila GSK-3 / shaggy enzyme, corntaining the phospho-Tyrosine 279 of h uman GSK-3α and phospho-Tyrosine 216 of human GSK-3β, respectively. This antibody does not react with GSK-3 or shaggy expressed in E. coli, but will react with the corresponding natural enzyme or the one expressed in Sf9 cells. The observed preference for the phosphorylated form is about 50:1.
The antigen has been described in the testis, thymus, prostate and ovary, weak in the brain, lung and kidney.

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