Product Description for Glycogen Synthase
Rabbit anti Human, Mouse Glycogen Synthase.
Product is tested for Western blot / Immunoblot.
Product is tested for Western blot / Immunoblot.
Properties for Glycogen Synthase
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Reactivity | Hu, Ms |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | Western Blot, The antibody has been used for Western blotting. No other applications have been tested. Recommended starting dilutions: Western Blot - 1:1000 *The optimal antibody concentration should be determined empirically for each specific application. , Western Blot |
| Background | Glycogen Synthase (GS), is an 85 kDa protein and a key enzyme in regulating glycogen synthesis by catalyzing the rate-limiting step of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Muscle glycogen synthase is phosphorylated on at least nine sites by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation. GSK-3 phosphorylates glycogen synthase at four serine sites in the COOH terminus including serines 641 and 645. Serine 641 is also phosphorylated by DYRK, while serine 645 is phosphorylated efficiently by SAPK2b/p38b. SAPK2b/p38b is believed to represent a priming kinase that allows GSK-3 to phosphorylate glycogen synthase at serine 641 and therefore inhibits its activity. |
| General Readings | 1. Takadera, T., & Ohyashiki, T. Glycogen synthase kinase-3 inhibitors prevent caspase-dependent apoptosis induced by ethanol in cultured rat cortical neurons. Eur. J. Pharmacol. 499(3):239-245, 2004. 2. Nielson, J.N. & Wojatszewski, J.F. Regulation of glycogen synthase activity and phosphorylation by exercise. Proc. Nutr. Soc. 63(2):233-237, 2004. 3. Ugi, S., et al. Protein phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (protein kinase B) activity in 3T3-L1 adipocytes. Mol. Cell Biol. 24(19):8778-8789, 2004. 4. Green, A.R., et al. Protein-targeting-to-glycogen promotes glycogen storage in hepatocytes by inactivation of phosphorylase and enzyme translocation. J. Biol. Chem. (e pub) 5. Kuma, Y., et al. Indentification of glycogen synthase as a new substrate for stress-activated protein kinase 2b/p38beta. Biochem J. 379(1): 133-139, 2004. 6. Skurat, A.V., & Dietrich, A.D. Phosphrylation of Ser640 in muscle glycogen synthase by DYRK family protein kinases. J. Biol. Chem. 279(4):2490-2498. Nielson, J.N. and J.F. Wojtaszewski (2004) Regulation of glycogen synthase activity and phosphorylation by exercise. Proc. Nutr. Soc. 63(2):233-237. Ugi, S. et al. (2004) Protein Phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (Protein Kinase B) activity in 3T3-L1 adipocytes. Mol. Cell. Biol. 24(19):8778-8789. Grenn, E.R. et al. (2004) Protein-targeting-to-glycogen promotes glycogen storage in hepatocytes by inactivation of phosphorylase and enzyme translocation. J. Biol. Chem. [Epub ahead of print] . Ugi, S. (2004) Protein Phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (Protein Kinase B) activity in 3T3-L1 adipocytes. Mol. Cell. Biol. 24(19):8778-8789. Green, A.R., et al. (2004) Protein-targeting-to-glycogen promotes glycogen storage in hepatocytes by inactivation of phosphorylase and enzyme translocation. J. Biol. Chem. [Epub ahead of print]. Kuma, Y., et al. (2004) Identification of glycogen synthase as a new substrate for stress-activated protein kinase 2b/p38beta. Biochem. J. 379(Pt 1):133-139. Skurat, A.V. and A.D. Dietrich (2004) Phosphorylation of Ser640 in muscle glycogen synthase by DYRK family protein kinases. J. Biol. Chem. 279(4):2490-2498. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Format |
|
| Specificity |
|
Accessory Products
Recommended Secondary Antibodies for Glycogen Synthase (9 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | AP | Rb | E, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | Rb | E, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | Rb | E, ICC/IF, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | Rb | E, P, WB | |||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | Rb | E, F, ICC/IF | |||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | Rb | F | |||
| Acris Antibodies GmbH | |||||||
| R1458C2 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy2 | |||||
| Acris Antibodies GmbH | |||||||
| R1458C3 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
|
Goat | Cy3 | |||||
| Acris Antibodies GmbH | |||||||
| R1458C5 |
Rabbit IgG (H+L chain, adsorbed) antibody |
||||||
|
Goat | Cy5 | |||||
| Acris Antibodies GmbH | |||||||
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
Over 280,000 products but you can't find the right antibody for your protein or application?
Acris will do the search for you!
