AM50278PU-N Glycoprotein hormones alpha chain antibody

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0.2 mg / €410.00
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Quick Overview

Mouse anti Human Glycoprotein hormones alpha chain HCGa/53

Product Description for Glycoprotein hormones alpha chain

Mouse anti Human Glycoprotein hormones alpha chain HCGa/53.
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Glycoprotein hormones alpha chain

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, FSH-alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, LH alpha, LSH-alpha, Luteinizing hormone alpha chain, Lutropin alpha chain, TSH-alpha, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, hCG-alpha
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone HCGa/53
Host Mouse
Isotype IgG1
Molecular weight ~13kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01215
Immunogen:
Recombinant hCG alpha protein.
GeneID:
1081
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use BSA free Antibody for coating.
Western Blotting: 0.5-1 µg/ml. 
Immunohistolochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Placenta.
Background HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
Concentration 0.2 mg/ml
General Readings
  1. McDonald EA, Wolfe MW. Adiponectin attenuation of endocrine function within human term trophoblast cells. Endocrinology. 2009 Sep;150(9):4358-65. doi: 10.1210/en.2009-0058. Epub 2009 Jun 11. PubMed PMID: 19520781.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Proten A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This MAb reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
Cellular Localization: Cytoplasmic, secreted.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for Glycoprotein hormones alpha chain (6 products)

Catalog No. Species Pres. Purity   Source  

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human Purified >98% pure (SDS-PAGE) Pregnancy Urine
  Acris Antibodies GmbH

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human Purified > 95 % Pituitary glands
  Acris Antibodies GmbH

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Glycoprotein hormones alpha chain

Glycoprotein hormones alpha chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Glycoprotein hormones alpha chain (2 products)

Catalog No. Species Pres. Purity   Source  

CGA 293T Cell Transient Overexpression Lysate(Denatured)

CGA 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

hCG alpha Lysate

Western Blot: Glycoprotein Hormones, alpha subunit Lysate [NBL1-09130] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CGA
  Novus Biologicals Inc.
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