discontinued

AM12128PU-S Glypican-3 / GPC3 (C-term) antibody

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Rabbit anti Human Glypican-3 / GPC3 SP86

AM12128PU-S

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Product Description for Glypican-3 / GPC3

Rabbit anti Human Glypican-3 / GPC3 SP86.
Properties: (C-term)
Presentation: Purified
Product is tested for Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Glypican-3 / GPC3

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms GTR2-2, Intestinal protein OCI-5, MXR7, OCI5
Presentation Purified
Reactivity Hu
Applications F, P, WB
Clonality Monoclonal
Clone SP86
Host Rabbit
Isotype IgG
Molecular weight 64 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P51654
Immunogen:
A synthetic peptide derived from C-terminus of Human Glypican-3 protein
GeneID:
2719
Remarks:
Epitope: C-terminus.
Property C-term
Isotype control PP501P
Application Western Blot: 1/25 for 1h at RT. 
Positive Control: HEPG2 Cell lysate.
FACS Analysis:
1/100.
Positive Control: HEPG2 Cell Line.
Immunohistochemistry on Paraffin Sections:
1/100.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Incubation Time: 30 minutes at RT.
Positive Control: Placenta, liver carcinoma.
Background Glypican-3 (GPC3) is a cell surface proteoglycan that bears heparan sulfate. It may be involved in the suppression/modulation of growth in the predominately mesodermal tissues and organs.
Expression alteration of Glypican-3 (GPC3) is associated with several malignancies and has been identified as an overexpressed gene in hepatocellular carcinoma (HCC). Glypican-3 is a cell surface proteoglycan that bears heparan sulfate. It may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS, pH 7.6
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes Glypican-3. 
This antibody can be used to detect Glypican-3 in both normal and neoplastic tissue types. This antibody should not be used to differentiate hepatocellular carcinoma (HCC) from either normal liver or other types of liver disease.
Cellular Localization: Cytoplasm.
Gene ID 2719

Accessory Products

Proteins and/or Positive Controls

Proteins for Glypican-3 / GPC3 (4 products)

Catalog No. Species Pres. Purity   Source  

Glypican-3 / GPC3

Glypican-3 / GPC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Glypican-3 / GPC3

Glypican-3 / GPC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Glypican-3 / GPC3

Glypican-3 / GPC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Glypican-3 / GPC3

Glypican-3 / GPC3 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.
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