AM33312PU-N Golgi Complex (Marker for Human Cells) antibody

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0.2 mg / €390.00
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Quick Overview

Mouse anti Human Golgi Complex 371-4

Product Description for Golgi Complex

Mouse anti Human Golgi Complex 371-4.
Properties: (Marker for Human Cells)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot, Flow Cytometry, Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Golgi Complex

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Golgi Complex, Golgi apparatus, Golgi zone
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone 371-4
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
SU-DHL-1 large cell lymphoma cells.
Property Marker for Human Cells
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blotting: 0.5-1.0 µg/ml.
Flow Cytometry: 0.5-1 µg/million cells in 0.1 ml. 
Immunofluorescence: 0.5-1 µg/ml. 
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 µg/ml for 30 minutes. 
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.  
Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control: HepG2, A431 or HeLa cells, Placenta, Testis, Tonsil or lymph node.
Concentration 0.2 mg/ml
General Readings
  1. Yuasa K, Omori K, Yanaka N. Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha. J Biol Chem. 2000 Feb 18;275(7):4897-905. PubMed PMID: 10671526.
  2. Endo Y, Obata T, Murata D, Ito M, Sakamoto K, Fukushima M, et al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer Sci. 2007 Oct;98(10):1633-7. Epub 2007 Aug 16. PubMed PMID: 17711502.
  3. Nakamura N, Rabouille C, Watson R, Nilsson T, Hui N, Slusarewicz P, et al. Characterization of a cis-Golgi matrix protein, GM130. J Cell Biol. 1995 Dec;131(6 Pt 2):1715-26. PubMed PMID: 8557739. (Free PMC Article available)
  4. Nakamura N, Lowe M, Levine TP, Rabouille C, Warren G. The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner. Cell. 1997 May 2;89(3):445-55. PubMed PMID: 9150144.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human.Does not react with Mouse or Rat.
Specificity
Specificity:
This Monoclonal Antibody Clone 371-4 recognizes an antigen associated with the Golgi complex in Human cells. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This Monoclonal Antibody is an excellent marker for Human cells in xenographic model research. It reacts specifically with Human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
Cellular LocalizationGolgi complex in cytoplasm. 
Negative Species: Mouse, Rat.

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