AM32065PU-N Golgi zone antibody

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0.1 mg / €300.00
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Quick Overview

Mouse anti Human Golgi zone AE-6

AM32065PU-N

Product Description for Golgi zone

Mouse anti Human Golgi zone AE-6.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections.

Properties for Golgi zone

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Golgi Body, Golgi Marker
Presentation Purified
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone AE-6
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
A BALB/c mouse was immunized with Su-DHL-1 large lymphoma cells.
The isolated splenocytes were fused with mouse myeloma cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry.
Immunofluorescence.

Immunohistochemistry on Frozen and Paraffin Sections: AE-6 produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and can be used for paraformaldehyde fixed or frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Recommended Positive Control: Tonsil.
Background The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region, and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells. Golgi markers are important in biology research as they aid in the behavioral and functional analysis of this dynamic organelle.
Concentration 0.1 mg/ml
General Readings This clone has not been published but similarly generated and characterized clones are described in:
1. Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using
monoclonal antibodies. In: Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
The Monoclonal antibody AE-6 recognizes an antigen found in the golgi zone of Human cells.
It can also be used as a marker of the golgi zone in subcellular fractions.

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