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AM32416PU-N gp200 renal cell carcinoma marker antibody

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0.1 mg / €300.00

Quick Overview

Mouse anti Human gp200 renal cell carcinoma marker PN-15

Product Description for gp200 renal cell carcinoma marker

Mouse anti Human gp200 renal cell carcinoma marker PN-15.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Frozen Sections, Paraffin Sections.

Properties for gp200 renal cell carcinoma marker

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone PN-15
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Renal cortical tissue extract.
Splenocytes were fused with mouse myeloma SP2/0Ag14 cells.
Isotype control AM03110PU-N, SM12P
Application Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: Use at 1/100-1/200 in an ABC method. 
Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. 
Suggested Incubation Time: 30 minutes at room temperature. 
Positive Control: Normal kidney or Renal Cell Carcinoma.
Background

gp200 is a surface membrane glycoprotein expressed on human embryonal carcinoma and is a malignant stem cell of testicular tumors. In normal kidney, gp200 is localised along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localised along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty one other normal tissues do not express similar or cross reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.

Concentration 0.1 mg/ml
General Readings
  1. Avery et al. Am. J. Surg. Pathol. 24: 203 (2000).
  2. Yoshida, S.O. and Imam, A; Cancer Res. 49; 1802-1809 (1989).
Storage

Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.

Format
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is useful in differential diagnosis of carcinomas. 
PN-15
reacts with a glycoprotein of 200 kD (gp200), present in proximal renal tubules.
The antigen is carbohydrate in nature and retained in formalin fixed paraffin embedded tissues. Other nornal tissues that display the antigen include breast, parathyroid glands and epididymis. Among renal carcinomas 93% of primary and 84% of metastatic carcinomas are positive. Relatively few other tumor types are postivie: breast cancers, teratocarcinomas and parathyroid adenomas. Protease digestion prior to antibody incubation enhances staining of tissue sections. 
Cellular Localization: Membrane.

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