AM32838PU-N Granulocyte antibody

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Mouse anti Human, Monkey Granulocyte BM-2

Product Description for Granulocyte

Mouse anti Human, Monkey Granulocyte BM-2.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections, Paraffin Sections.

Properties for Granulocyte

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu, Mky
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone BM-2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Nuclei from pokeweed mitogen stimulated Human peripheral blood lymphocytes.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 µg/106 cells.  
Immunofluorescence: 1-2 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed 
Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
No special pretreatment is required for staining of formalin/paraffin tissues. 
Recommended Positive Control: Tonsil or lymph node.
Background Granulocytes are nucleated white blood cells that have been classified according to the staining behavior of their cytoplasmic granules as neutrophils, eosinophils, or basophils. They are derived from the myeloid precursor series in the bone marrow and have a life span of only a few hours in peripheral blood. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. Eosinophils normally comprises about 3%, basophils comprise about 0.5% and neutrophils comprise about 60-80% of circulating WBCs. Eosinophils have large eosinophilic cytoplasmic granules and are prominent in parasitic infections and allergic reactions. Basophils have large basophilic granules that obscure the nucleus and are involved in type I hypersensitivity reactions. Neutrophils have a multilobate nucleus (for this reason they are also called polymorphonuclear leukocytes) and fine cytoplasmic granules. They participate in the nonspecific acute inflammatory response to injury. They are particularly active against extracellularly multiplying infectious agents, notably bacteria, but are also involved in repair and immune responses.
Until recently, immunological markers for myeloid cells have been lacking, especially those which identify different levels of cellular differentiation. The BM series provides a new panel of monoclonal antibodies which stain early precursor and mature forms of human myeloid cells. This panel of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemia's of similar derivation.
Concentration 0.2 mg/ml
General Readings
  1. Müller KM, Jaunin F, Masouyé I, Piguet PF, Saurat JH, Hauser C. Involvement of granulocytes and the adhesion receptors intercellular adhesion molecule-1 and lymphocyte function-associated antigen-1 in tissue inflammation induced by Th2-type helper cells. J Invest Dermatol. 1995 Mar;104(3):350-4. PubMed PMID: 7861000.
  2. Tinchon C, Auner HW, Beham-Schmid C, Aberle SW, Sill H, Linkesch W. Symptomatic Epstein-Barr virus reactivation in a patient with acute myeloid leukaemia and treatment with the monoclonal anti-CD20 antibody rituximab. Eur J Haematol. 2002 Jul;69(1):50-3. PubMed PMID: 12270062.
  3. de Swart RL, Ludlow M, de Witte L, Yanagi Y, van Amerongen G, McQuaid S, et al. Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. PLoS Pathog. 2007 Nov;3(11):e178. PubMed PMID: 18020706. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
Human and Macaque Monkey.
This antibody recognizes an unidentified antigen in the cytoplasm of mature Granulocytes. It shows no reactivity with any other cell type in Human tissues.
Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Granulocyte-specific BM-1 and BM-2 (Cat.-No AM32838PU) antibodies react with early precursor and mature forms of Human myeloid cells.
The Granulocyte BM-2 antibody (Cat.-No AM32838PU) is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a granulocyte marker in normal tissues or inflammatory processes.
Cellular LocalizationCytoplasmic.

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