AP31481SU-N Guinea Pig Serum Proteins antibody

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1 ml / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Goat anti Guinea Pig Guinea Pig Serum Proteins

AP31481SU-N

Product Description for Guinea Pig Serum Proteins

Goat anti Guinea Pig Guinea Pig Serum Proteins.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation, Radioimmunoassay.

Properties for Guinea Pig Serum Proteins

Product Category Primary Antibodies
Quantity 1 ml
Presentation Serum
Reactivity GP
Applications ID, IP, R
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Pooled whole Guinea Pig serum and partly purified serum fractions.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component.
To evaluate the purity of an isolated serum protein including immunoglobulins.
Since Immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by Immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar Nordic Nr. 2 contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar (Agar Nr. 1) with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 μl serum or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Lyophilized (delipidated, heat inactivated), stable whole antiserum without preservatives.
Serum
Reconstitution:
Restore by adding 1 ml sterile distilled water
Specificity
Specificity:
In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 18 individual proteins components.
Cross-reactivity.
Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.

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