AS07 260 H+ATPase (Plasma Membrane) antibody

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50 µl / €300.00
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Rabbit anti Arabidopsis H+ATPase (Plasma Membrane)

AS07 260

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  • AS07 260

Product Description for H+ATPase (Plasma Membrane)

Rabbit anti Arabidopsis H+ATPase (Plasma Membrane).
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for H+ATPase (Plasma Membrane)

Product Category Primary Antibodies
Quantity 50 µl
Synonyms Plasma Membrane Hydrogen ATPase
Presentation Aff - Purified
Reactivity Arabidopsis, conifers, di and monocots, ferns, mosses and green algae
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 95 kDa (Arabidopsis thaliana)
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer AgriSera AB

Datasheet Extract

Immunogen
Immunogen:
KLH-conjugated synthetic peptide exposed to cytoplasm in H+ATPase model, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR:At2g18960) and ATPase 2 (UniProt: P19456 , TAIR:At4g30190),3 (UniProt: P20431, TAIR:At5g57350),4 (UniProt: Q9SU58, TAIR:At3g47950),6 (UniProt: Q9SH76, TAIR:At2g07560),7 (UniProt: Q9LY32, TAIR:At3g60330),8 (UniProt: Q9M2A0, TAIR:At3g42640),9 (UniProt: Q42556, TAIR:At1g80660), 11 (UniProt: Q9LV11, TAIR:At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3).
Add. information cellular [compartment marker] for plasma membrane
Application Western blot (WB), immunofluorescence (IF), immunolocalization (IL). 
Recommended dilution: 
1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF), 1: 100 (IL).
For additional Western blot detection image please refer to the article below.
VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.


Background The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Product Citations
Originator or purchased from resellers:
  1. Berthier et al. (2014).Identification of a new sucrose transporter in rye-grass (LpSUT2): Effect of defoliation and putative fructose sensing. Plant Physiol & Biochem, vol: 84, Nov. 2014. (immunolocalization, leaf sheath)
  2. Hu X, Makita S, Schelbert S, Sano S, Ochiai M, Tsuchiya T, et al. Reexamination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Mar;167(3):660-70. doi: 10.1104/pp.114.252023. Epub 2015 Jan 12. PubMed PMID: 25583926. (Free PMC Article available, 7 images available)
  3. Kolb C, Nagel MK, Kalinowska K, Hagmann J, Ichikawa M, Anzenberger F, et al. FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis. Plant Physiol. 2015 Apr;167(4):1361-73. doi: 10.1104/pp.114.253377. Epub 2015 Feb 19. PubMed PMID: 25699591. (Free PMC Article available)
  4. Komatsu A, Terai M, Ishizaki K, Suetsugu N, Tsuboi H, Nishihama R, et al. Phototropin encoded by a single-copy gene mediates chloroplast photorelocation movements in the liverwort Marchantia polymorpha. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5. PubMed PMID: 25096976. (Free PMC Article available, 10 images available)
  5. Li ZG, Chen HW, Li QT, Tao JJ, Bian XH, Ma B, et al. Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis. Sci Rep. 2015 Jul 24;5:12477. doi: 10.1038/srep12477. PubMed PMID: 26207341. (Free PMC Article available, 9 images available)
  6. Migocka M, Papierniak A, Maciaszczyk-Dziubinska E, Posyniak E, Kosieradzka A. Molecular and biochemical properties of two P1B2-ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2015 Jun;38(6):1127-41. doi: 10.1111/pce.12447. Epub 2014 Oct 27. PubMed PMID: 25210955.
  7. Shen H, He Z, Yan H, Xing Z, Chen Y, Xu W, et al. The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Jun 13;105:46-57. doi: 10.1016/j.jprot.2014.01.029. Epub 2014 Feb 4. PubMed PMID: 24508335.
  8. Zhang K, Novak O, Wei Z, Gou M, Zhang X, Yu Y, et al. Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nat Commun. 2014;5:3274. doi: 10.1038/ncomms4274. PubMed PMID: 24513716.
General Readings

Kolb et al. (2015). FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana. Plant Physiol. 2015 Feb 19. pii: pp.114.253377. 

Hu et al. (2015). Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Jan 12. pii: pp.114.252023.
Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5.
Berthier et al. (2014).Identification of a new sucrose transporter in rye-grass (LpSUT2): Effect of defoliation and putative fructose sensing. Plant Physiol & Biochem, vol: 84, Nov. 2014. (immunolocalization, leaf sheath)
Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.
Shen
et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.
Zhang et al. (2014). Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nat Commun. 2014 Feb 11;5:3274. doi: 10.1038/ncomms4274.
Sobrino-Plata
et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Do not store this antibody in 4°C.

Format
State:
rabbit polyclonal serum lyophilized
Aff - Purified
Reconstitution:
50 µl lyophilized for reconstitution add 50 µl of sterile water
Species Reactivity
Species reactivity (expected):
dicots including: Mesembruanthemum crystallinum, Solanum tuberosum; monocots (including Avena sativa, Hordeum vulgare, conifers (Pinus thunbergii), mosses (Physocomitrella patens), algae (Dunaliella spp., Ostreococcus spp.), Saccharomyces cerevisiae.
Species reactivity (tested):
dicots including: Arabidopsis thaliana, Cucumis sativus, Cucurbita moschata, Glycine max (weak), Kandelia obovata, Lolium perenne, Lycopersicon esculentum, Marchantia polymorpha, Medicago truntata, Nicotiana tabacum, Noccaea caerulescens, Phalenopsis Sogo Yukidian cultivar V3, Ricinus communis, Spinacia oleracea, monocots inlcuding: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea abies, Populus tremula, Pteris vittata (fern), algae: Chlamydmonas reinhardtii. not reactive in Aspergillus niger
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