AM20783PU-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 ml / €240.00
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Mouse anti All Species, Human HA Epitope Tag (YPYDVPDYA) 26D11

AM20783PU-N

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Product Description for HA Epitope Tag (YPYDVPDYA)

Mouse anti All Species, Human HA Epitope Tag (YPYDVPDYA) 26D11.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation Aff - Purified
Reactivity All Species, Hu
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone 26D11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide containing the Infuenza Hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
Add. information Use an anti-Mouse secondary antibody to detect the 26D11 antibody.
Application ELISA: 1/2000.
Western blot: 1/5000.
Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1 TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1/100.
Immunofluorescence: 1/1000.
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
General Readings
  1. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlon, P.J. 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6: 1204-1210.
  2. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31-40. PubMed PMID: 3047011.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody detects over-expressed proteins containing the HA epitope tag.

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