AP00533PU-N HA Epitope Tag (YPYDVPDYA) antibody

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Rabbit anti HA Epitope Tag (YPYDVPDYA)


Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.5 mg
Presentation Purified
Applications IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic peptide (HA-tag)
AA Sequence:
Application Western Blotting (5-10 µg/ml for 2hrs at RT; Recommended positive control: Proteins displaying the YPYDVPDYA HA-tag).
Immunoprecipitation (10 µg/mg protein lysate; Use Protein A).
Background A small segment of the viral hemagglutinin coat protein is widely used as an epitope tag to facilitate purification and functional analysis of proteins of interest. Epitope tags are short peptide sequences that are easily recognized by readily available tag-specific antibodies. Ligation of epitope-tag encoding DNA to cloned DNAs of interest produces epitope tagged fusion proteins that can then be subjected to antibody-dependent experimental procedures. Epitope tagging techniques are widely used in the purification, identification, and functional analysis of proteins. Expression vectors for producing tag fusion proteins in a variety of organisms (including bacteria, yeast, insect, and mammalian cells) are commercially available. Some of the more commonly used fusion tags include: cmyc, GFP, GST, HA, His, amd MBP.
Concentration 1.0 mg/ml
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Liquid purified IgG fraction
Proteins displaying the YPYDVPDYA HA-tag.
Cellular localization depends upon the localization of the parent protein tagged with the HA tag.

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