AP05576PU-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 mg / €540.00
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Rabbit anti HA Epitope Tag (YPYDVPDYA)

AP05576PU-N

Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
AA Sequence:
YPYDVPDYA
Application Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Citrate buffer is recommended for this purpose).
ELISA.
Western blot.
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Concentration 0.5 mg/ml
General Readings
  1. Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, et al. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol. 1988 May;8(5):2159-65. PubMed PMID: 2455217. (Free PMC Article available)
  2. Wilson IA, Niman HL, Houghten RA, Cherenson AR, Connolly ML, Lerner RA. The structure of an antigenic determinant in a protein. Cell. 1984 Jul;37(3):767-78. PubMed PMID: 6204768.
Storage Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Format
Buffer System:
0.02M Potassium phosphate, 0.15M Sodium chloride pH7.2, 0.01% Sodium Azide (NaN3)
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors.
The antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.

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