AP09230BT-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 mg / €440.00
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Rabbit anti HA Epitope Tag (YPYDVPDYA)

Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Biotin
Applications E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide
Remarks:
A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Application ELISA: 1:15,000 - 1: 150,000.
Immunohistochemistry on paraffin sections: 1:250.
Immunoprecipitation: 1:250.
Western blot: 1:10,000 (In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins).
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings (Conjugation) Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Storage Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Sodium Azide
State:
Liquid sterile filtered Ig fraction
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors. It is directed against the HA motif and is useful in determining its presence in various assays. Anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.

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