AP09230D8-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 mg / €360.00

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Rabbit anti HA Epitope Tag (YPYDVPDYA)

Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: IRDYE800
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation IRDYE800
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide
A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Application Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye(TM)800 antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. IRDye(TM)800 conjugates are optimized for the Odyssey® Infrared Imaging System developed by LI-COR. IRDye(TM)800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when IRDye(TM)800 conjugates are used in conjunction with Cy5.5(TM) conjugates. IRDye(TM)800 conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. Anti-HA is optimally suited for monitoring the expression of HA tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- terminus of targeted proteins as is expressed in many commonly used expression vectors.
Western blot: 1:10,000 to 1:25,000 is suggested for this product to detect 12-25 pg of immobilized protein.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The antiepitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings (Conjugation) LI-COR Biosciences, Lincoln, NE.
Storage Store vial (undiluted) at 2-8 ° C. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
IRDye(TM)800 (MW 1166.2)
Absorption emission:
774 nm (in PBS) / 800 nm
Molar radio:
3.0 moles IRDye(TM)800/mole of Rabbit IgG
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml BSA IgG and Protease free; 0.01% (w/v) Sodium Azide
Lyophilized Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This antibody is directed against HA epitope tag and is useful in determining its presence in various assays. Anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. The antibody recognizes the HA-tag (Tyr-Pro-Tyr- Asp-Val-Pro-Asp-Tyr-Ala) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

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