discontinued

AP09230DL4-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 mg / €400.00

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Rabbit anti HA Epitope Tag (YPYDVPDYA)

AP09230DL4-N

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  • AP09230DL4-N

Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: DyLight405
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation DyLight405
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding aa 114-122 of hemagglutinin influenza conjugated to KLH using maleimide
AA Sequence:
Y-P-Y-D-V-P-D-Y-A-G
Remarks:
Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester
Add. information Instrument compatibility: The emission spectra for this DyLight(TM) conjugate match the principal output wavelengths of most common fluorescence instrumentation.
Application Flourescenct Western blot: > 1:20,000.
ELISA (FLISA): > 1:10,000.
Immunoflourescence: > 1:5,000.
This antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The antiepitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
Format
Label:
DyLightTM 405 (MW 793)
DyLight405
Absorption emission:
400 nm (in PBS) / 400 nm (in PBS)
Molar radio:
2.0 moles DyLight(TM) 405 per mole of Rabbit IgG
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml BSA, IgG and Protease free; 0.01% (w/v) Sodium Azide
State:
Lyophilized Ig fraction
Reconstitution:
Rehydrate with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody is directed against the HA epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the HA epitope tag (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Gly) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

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