discontinued

AP09230DL5-N HA Epitope Tag (YPYDVPDYA) antibody

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0.1 mg / €400.00

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Rabbit anti HA Epitope Tag (YPYDVPDYA)

AP09230DL5-N

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  • AP09230DL5-N

Product Description for HA Epitope Tag (YPYDVPDYA)

Rabbit anti HA Epitope Tag (YPYDVPDYA).
Presentation: DyLight488
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation DyLight488
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding aa 114-122 of Hemagglutinin Influenza conjugated to KLH using maleimide.
AA Sequence:
Y-P-Y-D-V-P-D-Y-A-G
Remarks:
Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester
Add. information Instrument compatibility: The emission spectra for this DyLightTM conjugate match the principal output wavelengths of most common fluorescence instrumentation.
Application ELISA (FLISA): > 1/20,000.
Western blot: 1/00,000-1/25,000.
Immunoflourescence: > 1/5,000.
This antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The antiepitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store vial at 2-8°C prior to restoration. Following restoration product can be stored undiluted at 2-8°C for one month or (in aliquots) at -20°C or below.
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.
Format
Label:
DyLight(TM) 488 (MW 1,011.20)
DyLight488
Molar radio:
4.0 DyLightTM 488 per mole of Rabbit IgG
Purification:
Affinity Chromatography.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody is directed against the HA epitope tag and is useful in determining its presence in over expressed proteins in various assays.
The antibody recognizes the HA epitope tag (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Gly) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

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