AP31789FC-N HA Epitope Tag (YPYDVPDYA) antibody

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1 ml / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Chicken anti HA Epitope Tag (YPYDVPDYA)


Product Description for HA Epitope Tag (YPYDVPDYA)

Chicken anti HA Epitope Tag (YPYDVPDYA).
Presentation: FITC
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 1 ml
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation FITC
Applications C, ICC/IF, WB
Clonality Polyclonal
Host Chicken
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Synthetic peptide containing the Influenza Hemaglutinin epitope (YPYDVPDYA) conjugated to Keyhole Limpet Hemocyanin (KLH). After repeated injections, immune eggs were collected, from which the IgY fractions were prepared.
Application ELISA.
Western Blot.
Quality Control: Antibodies were analyzed using ELISA and western blot analyses prior to labeling with Fluorescein. ELISA analysis involved plates coated with peptide conjugated to a different carrier protein.
Detection of the Chicken anti-HA antibodies was accomplished using HRP-labeled Goat anti-Chicken IgY at a dilution of 1/10,000. Under these conditions, the unlabelled Chicken anti-HA antibodies (diluted 1/10,000) produced an optical density of > 1.0 in a 15 minute reaction using Tetramethylbenzidine as the substrate.
By western blot, the unlabeled antibodies were diluted 1/1,000 and gave a prominent band using HRP-labeled Goat anti-Chicken IgY at a dilution of 1/10,000.
Concentration 0.1 mg / ml (based on absorbance at 280 nm)
Storage Store the antibody undiluted in the dark at 2-8°C.
Shelf life: one year from despatch.
Absorption emission:
Ratio of absorbance at 495/280 = 0.88
Molar radio:
F/P = 3.6
Affinity Chromatography using a peptide column.
Buffer System:
PBS, pH 7.2 containing 0.2% BSA as stabilizer and 0.02% Sodium Azide as preservative.
Liquid purified (0.45 μm filter sterilized) IgY fraction.
HA (Influenza Hemaglutinin) epitope tag.

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