AP31794AG-N HA-PrecipHen® HA Epitope Tag (YPYDVPDYA) antibody

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2 ml / €410.00

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Chicken anti HA-PrecipHen® HA Epitope Tag (YPYDVPDYA)

Product Description for HA-PrecipHen® HA Epitope Tag (YPYDVPDYA)

Chicken anti HA-PrecipHen® HA Epitope Tag (YPYDVPDYA).
Presentation: Agarose
Product is tested for Immunoprecipitation.

Properties for HA-PrecipHen® HA Epitope Tag (YPYDVPDYA)

Product Category Primary Antibodies
Quantity 2 ml
Synonyms HA Tag, HA-Tag, Hemagglutinin Tag
Presentation Agarose
Applications IP
Clonality Polyclonal
Host Chicken
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immune sera was collected from chicken that were hyper-immunized with KLH coupled with a synthetic peptide containing the HA epitope tag (YPYDVPDYA).
Chicken IgY antibodies were purified from egg yolks, and then affinity purified using the conate peptide coupled to Agarose. After exhaustive washes with Sodium Phosphate-Buffered (10 mM, pH 7.2) isotonic Saline (PBS), the IgY-coupled Agarose beads were suspended in PBS with 0.02% Sodium Azide .
Application Immunoprecipitation.
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
Concentration 2.0 ml bed volume in a total of 4.0 ml
Storage Store the antibody at 4°C in the dark.
Avoid repeated freezing and thawing.
Centrifuge vial before opening.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
50mM HEPES, pH 7.6, 5mM EDTA containing 0.02% Sodium Azide as preservative.
Agarose beads in buffer
Recombinant proteins with HA epitope tags.
Binding Capacity: 1.2 mg of HA-tagged Ovalbumin per ml bed volume.
Quality control: The capacity of PrecipHen® was determined by measuring how much HA-tagged Ovalbumin bound to 1.0 ml bed volume of Agarose. After overloading the gel, the Agarose was washed, and the HA-Ovalbumin was eluted.
To meet quality control standards, the capacity of the HA-PrecipHen needs to be at least 1.0 mg of HA-Ovalbumin.

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