TA327260 HCK antibody

HCK Rabbit Polyclonal Antibody

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0.1 mg / €335.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat HCK

Product Description for HCK

Rabbit anti Human, Mouse, Rat HCK.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for HCK

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms B-cell/myeloid kinase, BMK, Hemopoietic cell kinase, Tyrosine-protein kinase HCK, p56-HCK, p59-HCK, p60-HCK
Presentation Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P08631
Immunogen:
Recombinant protein of human HCK.
GeneID:
3055
Application WB: 1:500-1:2000; IHC: 1:50-1:200
Background Hck (hemopoietic cell kise) is a protein tyrosine kise of the Src family prominently expressed in the lymphoid and myeloid lineages of hemopoiesis. It participates in transducing a variety of extracellular sigls, which ultimately affect cellular processes including proliferation, differentiation and migration.The well-defined modular structure of Hck comprises a relatively divergent, NH2-termil "unique" domain, which is subject to post-translatiol lipid modifications thereby targeting Hck to the plasma membrane. Src homology 3 (SH3) and 2 (SH2) domains, and a tyrosine kise catalytic domain follow the "unique" domain. The catalytic activity of Hck is regulated, both positively and negatively, by tyrosine phosphorylation of highly conserved tyrosine (Y) residues. Phosphorylation of a single conserved Tyr499 residue in the COOH terminus of Hck by the protein kise Csk renders Hck ictive as a result of an intramolecular interaction between the phosphorylated tyrosine (pY) residue and its own SH2 domain. Disruption of this interaction, either as a result of dephosphorylation, or substitution of the COOH-termil regulatory Y residue with phenylalanine (F; e.g., HckY499F), or COOH-termil truncation mutations as observed in the virally transduced v-Src oncoprotein, results in constitutive activation of Hck. In contrast to phosphorylation of the COOH-termil regulatory tyrosine residue, autophosphorylation of a tyrosine residue (Tyr388) within the kise domain of Hck acts to positively regulate its catalytic activity. Thus, activation of Hck requires both disruption of the COOH-termil regulatory tyrosine-SH2 domain interaction and autophosphorylation of the regulatory tyrosine residue within the kise domain. The dysfunction or dysregulation of Hck may contribute to the pathogenesis of some human leukemias.
Format
Purification:
Affinity purification
Buffer System:
Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
State:
Purified

Accessory Products

Proteins and/or Positive Controls

Proteins for HCK (9 products)

Catalog No. Species Pres. Purity   Source  

HCK

HCK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HCK

HCK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

HCK

HCK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

HCK

HCK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

HCK

HCK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

HCK

HCK Human
  Abnova Taiwan Corp.

HCK

HCK Human
  Abnova Taiwan Corp.

HCK

HCK Human
  Abnova Taiwan Corp.

HCK

HCK Human
  Abnova Taiwan Corp.

Positive controls for HCK (3 products)

Catalog No. Species Pres. Purity   Source  

HCK 293T Cell Transient Overexpression Lysate(Denatured)

HCK 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

HCK overexpression lysate

HCK overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

HCK overexpression lysate

HCK overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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