DM3097 Heat Shock Protein 70 (HSP70) antibody

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Mouse anti Bovine, Human, Monkey Heat Shock Protein 70 (HSP70) W27


Product Description for Heat Shock Protein 70 (HSP70)

Mouse anti Bovine, Human, Monkey Heat Shock Protein 70 (HSP70) W27.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Heat Shock Protein 70 (HSP70)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HSP-70, HSP70-1, HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat Shock 70 kDa Protein 1
Presentation Purified
Reactivity Bov, Hu, Mky
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone W27
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

HSP70 protein from HeLa Cells.
Isotype control AM03096PU-N
Application Flow Cytometry. Immunofluorescence. Immunoprecipitation (Native and denatured): Use Protein A; Ab 2µg/mg protein lysate. Western Blotting: Ab 0.5-1µg/ml for 2hrs at RT. Immunohistology (Formalin/paraffin): Use Ab 2-4µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0. Recommended positive control: BT474 or HeLa Cells, Breast Carcinoma. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Heat shock proteins (HSPs) or stress response proteins (SRPs) are synthesized in variety of environmental and pathophysiological stressful conditions. These proteins are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T-antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions. Reacts with Human, Monkey, and Cow. Others not tested. Cellular Localization: cytoplasmic, nuclear.

Notes: Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70

Mol. Wt. of Antigen: 73kD

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