NB120-5442 Heat Shock Protein 70 (HSP70) antibody

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Mouse anti Human, Mouse, Rat, Yeast Heat Shock Protein 70 (HSP70) 2A4


Product Description for Heat Shock Protein 70 (HSP70)

Mouse anti Human, Mouse, Rat, Yeast Heat Shock Protein 70 (HSP70) 2A4.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Heat Shock Protein 70 (HSP70)

Product Category Primary Antibodies
Quantity 0.05 ml
Synonyms HSP-70, HSP70-1, HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat Shock 70 kDa Protein 1
Presentation Ascites
Reactivity Hu, Ms, Rt, Ye
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone 2A4
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Human recombinant Hsp 70 over expressed in E. coli.
Application immunofluorescence, Immunohistochemistry-Paraffin, Western Blot, IHC-P: Antigen retrieval is not essential but may optimise staining. Two-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., immunofluorescence 1/100, Western Blot 1/5000, Immunohistochemistry-Paraffin
Background The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria.
General Readings General / background references:Carr VM et al. Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein. J Comp Neurol 348:150-60 (1994). PubMed PMID: 7814683
Milarski KL et al. Cell cycle-dependent association of HSP70 with specific cellular proteins. J Cell Biol 108:413-23 (1989). PubMed PMID: 2645297
Storage Store at -20C. Avoid freeze-thaw cycles.
Buffer System:
0.05% Sodium Azide
Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human Hsp 70., Detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70 and, following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. By Western blot, this antibody detects proteins from ~70 kDa to ~72 kDa representing different members of the HSP70 family.
Amphibian, Avian, Drosophila, Fish, Human, Mouse, Rat, Yeast

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